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Expression of RoTat 1.2 cross-reactive variable antigen type in Trypanosoma evansi and T. equiperdum

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dc.contributor.author Claes, F. en_US
dc.contributor.author Verloo, D. en_US
dc.contributor.author de Waal, D. T. en_US
dc.contributor.author Urakawa, T. en_US
dc.contributor.author Majiwa, P. en_US
dc.contributor.author Goddeeris, B. M. en_US
dc.contributor.author Büscher, P. en_US
dc.date.accessioned 2007-12-06T14:33:17Z
dc.date.available 2007-12-06T14:33:17Z
dc.date.issued 2002 en_US
dc.identifier.issn 0077-8923 en_US
dc.identifier.other ITG-P1A en_US
dc.identifier.other ITG-P2A en_US
dc.identifier.other ITG-PLA en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-SEROL en_US
dc.identifier.other JIF en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/100
dc.description.abstract The variable antigen type (VAT) RoTat 1.2 has been cloned from a T. evansi strain, isolated in 1982 from a water buffalo in Indonesia. All T. evansi isolates hitherto tested express this VAT. In a study on the differential diagnosis of T. equiperdum and T. evansi in horses, we investigated serological evidence for the expression of RoTat 1.2 in 11 T. evansi and six T. equiperdum populations originating from Asia, Europe, Africa, and the Americas. Preinfection sera and sera of days 7, 14, 25, and 35 post-infection (p.i.) were analyzed for the presence of antibodies reactive with RoTat 1.2 in immune trypanolysis, ELISA/T. evansi and CATT/T. evansi. Within the duration of the experiment, all rabbits infected with T. evansi became positive in the three serological tests. Five out of six rabbits infected with T. equiperdum also became positive in the three tests. Only one T. equiperdum strain (the OVI strain from South Africa) did not induce the production of antibodies reactive with RoTat 1.2 and thus might not contain or express a VSG that shares epitopes similar to those on the RoTat 1.2 VSG. The data lead to the conclusion that T. equiperdum can express VSGs containing epitopes serologically similar to those in the T. evansi RoTat 1.2 VAT. This explains, in part, why the antibody detection tests based on Ro Tat 1.2 VSG cannot reliably distinguish between the infections caused by T. evansi and those caused by T. equiperdum. There are no data that contradict the possibility that the putative T. equiperdum strains, which express VSGs with epitopes similar to those on RoTat 1.2, are actually T. evansi. en_US
dc.language English en_US
dc.subject Protozoal diseases en_US
dc.subject Trypanosoma evansi en_US
dc.subject Trypanosoma equiperdum en_US
dc.subject Surra en_US
dc.subject Dourine en_US
dc.subject Differential diagnosis en_US
dc.subject Antibody detection en_US
dc.subject RoTat 1.2 en_US
dc.subject Variable antigen type en_US
dc.title Expression of RoTat 1.2 cross-reactive variable antigen type in Trypanosoma evansi and T. equiperdum en_US
dc.type Article en_US
dc.citation.jtitle Annals of the New York Academy of Sciences en_US
dc.citation.volume 969 en_US
dc.citation.pages 174-179 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/12381586
dc.citation.jabbreviation Ann NY Acad Sci en_US


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