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Xpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis: a diagnostic evaluation study [Epub ahead of print]

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Show simple item record Tadesse, M. en_US Abebe, G. en_US Bekele, A. en_US Bezabih, M. en_US Yilma, D. en_US Apers, L. en_US de Jong, B. C. en_US Rigouts, L. en_US 2019-06-04T12:20:49Z 2019-06-04T12:20:49Z 2019 en_US
dc.identifier.issn 1198-743X en_US
dc.identifier.doi en_US
dc.identifier.other en_US
dc.identifier.other 6pp. en_US
dc.identifier.other ITG-B1B; ITG-C6A; ITG-B7A; ITG-BLA; MULTI; DBM; U-MYCOB; DCS; U-HIVCLI; JIF; DOI; PDF; Abstract; ITMPUB; DSPACE65 en_US
dc.description.abstract OBJECTIVES: The diagnosis of extrapulmonary tuberculosis (EPTB) is often made on clinical suspicion alone, resulting in both under- and over diagnosis and relatively poor outcomes. In this study, we evaluated the clinical utility of the Xpert MTB/RIF on routinely collected extra-pulmonary specimens in Ethiopia. METHODS: This study was carried out at Jimma University Specialized Hospital, Southwest Ethiopia. Extra-pulmonary specimens were collected from 572 patients clinically suspected of suffering from EPTB. All specimens were tested for TB by smear-microscopy, culture and Xpert MTB/RIF. The diagnostic accuracy of Xpert MTB/RIF was calculated compared to a composite reference standard (CRS), comprising clinical and laboratory results. RESULTS: In total, 572 extra-pulmonary specimens (279 lymph node, 159 pleural, 80 peritoneal, 45 cerebrospinal and 9 pericardial fluids) were tested. The pooled sensitivity and specificity of Xpert MTB/RIF were calculated to be 75% [95% CI: 70-80] and 98% [95% CI: 97-100] respectively when compared to CRS. The highest sensitivity was documented for lymph node specimen (90% [95% CI: 86-94]), moderate sensitivity for cerebrospinal fluid (53% [95% CI: 28-79), while the sensitivity was lowest for pleural (30% [95% CI: 17-44]) and peritoneal (32% [95% CI: 12-51]) fluids. Xpert MTB/RIF in addition detected rifampicin resistance in 13 patients, in perfect agreement with results from line probe assay. CONCLUSIONS: Xpert MTB/RIF may be used as initial diagnostic tool for testing of lymph node specimens from patients suspected of having TB lymphadenitis. The added value of Xpert MTB/RIF to diagnose pleural or peritoneal TB is limited by its poor sensitivity. en_US
dc.language English en_US
dc.relation.uri en_US
dc.subject Tuberculosis-extrapulmonary en_US
dc.subject Bacterial diseases en_US
dc.subject Diagnosis en_US
dc.subject Evaluation en_US
dc.subject Xpert MTB/RIF en_US
dc.title Xpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis: a diagnostic evaluation study [Epub ahead of print] en_US
dc.type Article-P en_US
dc.citation.jtitle Clinical Microbiology and Infection en_US
dc.citation.abbreviation Clin Microbiol Infect en_US

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