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Validation of a new experimental model for assessing drug efficacy against infection with Trypanosoma equiperdum in horses

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Show simple item record Hebert, L. en_US Guitton, E. en_US Madeline, A. en_US Geraud, T. en_US Carnicer, D. en_US Lakhdar, L. en_US Pitel, P. H. en_US Coste, M. en_US Laloy, E. en_US Giraudet, A. en_US Zientara, S. en_US Büscher, P. en_US Laugier, C. en_US Hans, A. en_US Petry, S. en_US Cauchard, J. en_US 2019-06-04T12:21:30Z 2019-06-04T12:21:30Z 2018 en_US
dc.identifier.issn 0304-4017 en_US
dc.identifier.doi en_US
dc.identifier.other en_US
dc.identifier.other ITG-B12A; DBM; U-MOLPAR; JIF; DOI; PDF; Abstract; ITMPUB; DSPACE65 en_US
dc.description.abstract Trypanosoma equiperdum, the causative agent of dourine, may affect the central nervous system, leading to neurological signs in infected horses. This location protects the parasite from most (if not all) existing chemotherapies. In this context, the OIE terrestrial code considers dourine as a non-treatable disease and imposes a stamping-out policy for affected animals before a country may achieve its dourine-free status. The use of practices as drastic as euthanasia remains controversial, but the lack of a suitable tool for studying a treatment's efficacy against dourine hampers the development of an alternative strategy for dourine infection management. The present study reports on the development of an experimental infection model for assessing drug efficacy against the nervous form of dourine. The model combines the infection of horses by Trypanosoma equiperdum and the search for trypanosomes in the cerebrospinal fluid (CSF) through an ultrasound-guided cervical sampling protocol. After a development phase involving four horses, we established an infection model that consists of inoculating 5 x 10(4)T. equiperdum OVI parasites intravenously into adult Welsh mares (Equus caballus). To evaluate its efficacy, eight horses were infected according to this model. In all these animals, parasites were observed in the blood at 2 days post-inoculation (p.i.) and in CSF (12.5 +/- 1.6 days p.i.) and seroconversion was detected (8.25 +/- 0.5 days p.i.). All eight animals also developed fever (rectal temperature > 39 degrees C), low hematocrit (< 27%), and ventral edema (7.9 +/- 2.0 days p.i.), together with other inconstant clinical signs such as edema of the vulva (six out of eight horses) or cutaneous plaques (three out of eight horses). This model provides a robust infection protocol that induces an acute trypanosome infection and that allows parasites to be detected in the CSF of infected horses within a period of time compatible with animal experimentation constraints. We conclude that this model constitutes a suitable tool for analyzing the efficacy of anti-Trypanosoma drugs and vaccines. en_US
dc.language English en_US
dc.relation.uri en_US
dc.subject Trypanosomiasis-animal en_US
dc.subject Trypanosoma equiperdum en_US
dc.subject Protozoal diseases en_US
dc.subject Animal diseases en_US
dc.subject Horses en_US
dc.subject Treatment en_US
dc.subject Efficacy en_US
dc.subject Experimental en_US
dc.title Validation of a new experimental model for assessing drug efficacy against infection with Trypanosoma equiperdum in horses en_US
dc.type Article en_US
dc.citation.jtitle Veterinary Parasitology en_US
dc.citation.volume 263 en_US
dc.citation.pages 27-33 en_US
dc.citation.abbreviation Vet Parasitol en_US

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