Institute of Tropical Medicine Antwerp
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16S metagenomics for diagnosis of bloodstream infections: opportunities and pitfalls

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dc.contributor.author Rutanga, J. P. en_US
dc.contributor.author Van Puyvelde, S. en_US
dc.contributor.author Heroes, A. S. en_US
dc.contributor.author Muvunyi, C. M. en_US
dc.contributor.author Jacobs, J. en_US
dc.contributor.author Deborggraeve, S. en_US
dc.date.accessioned 2019-06-04T12:22:02Z
dc.date.available 2019-06-04T12:22:02Z
dc.date.issued 2018 en_US
dc.identifier.issn 1473-7159 en_US
dc.identifier.doi http://dx.doi.org/10.1080/14737159.2018.1498786 en_US
dc.identifier.other ITG-B1B; ITG-B2A; ITG-C3A; ITG-C5A; ITG-BLA; MULTI; DBM; U-DIABAC; DCS; U-TLM; JIF; DOI; Abstract; Not available; ITMPUB; DSPACE65; NOBIT en_US
dc.identifier.uri http://hdl.handle.net/10390/10760
dc.description.abstract INTRODUCTION: Bacterial bloodstream infections (BSI) form a large public health threat worldwide. Current routine diagnosis is based on blood culture (BC) but this technique suffers from limited sensitivity. Molecular diagnostic tools have been developed for identification of bacteria in the blood of BSI patients. 16S metagenomics is an open-ended technique that can detect simultaneously all bacteria in a given sample based on PCR amplification of the 16S ribosomal RNA gene (rDNA) followed by sequencing of the PCR amplicons and taxonomic labeling of the sequence reads at genus or species level. Areas covered: Here we review the studies that have used 16S metagenomics for the identification of bacteria in human blood samples. We also discuss the potential added value of 16S metagenomics in the diagnosis of BSI, challenges as well as future directions for implementation in clinical settings. Expert commentary: 16S metagenomics has the potential to complement conventional BC; however, the technique currently suffers from several technical limitations jeopardizing implementation in routine clinical microbiology laboratories. Further studies are required to assess the cost-efficiency and clinical impact of 16S metagenomics in comparison to BC which remains the gold standard diagnostic method for BSI. en_US
dc.language English en_US
dc.relation.uri http://www.ncbi.nlm.nih.gov/pubmed/29985081 en_US
dc.subject Blood-borne diseases en_US
dc.subject Diagnostic tests en_US
dc.title 16S metagenomics for diagnosis of bloodstream infections: opportunities and pitfalls en_US
dc.type Article en_US
dc.citation.issue 8 en_US
dc.citation.jtitle Expert Review of Molecular Diagnostics en_US
dc.citation.volume 18 en_US
dc.citation.pages 749-759 en_US
dc.citation.abbreviation Expert Rev Mol Diagn en_US


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