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Re-visiting the detection of porcine cysticercosis based on full carcass dissections of naturally Taenia solium infected pigs

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Show simple item record Chembensofu, M. en_US Mwape, K. E. en_US Van Damme, I. en_US Hobbs, E. en_US Phiri, I. K. en_US Masuku, M. en_US Zulu, G. en_US Colston, A. en_US Willingham, A. L. en_US Devleesschauwer, B. en_US Van Hul, A. en_US Chota, A. en_US Speybroeck, N. en_US Berkvens, D. en_US Dorny, P. en_US Gabriel, S. en_US 2019-06-04T12:22:21Z 2019-06-04T12:22:21Z 2017 en_US
dc.identifier.issn 1756-3305 en_US
dc.identifier.doi en_US
dc.identifier.other en_US
dc.identifier.other 9 pp. en_US
dc.identifier.other 26 en_US
dc.identifier.other ITG-B4A; ITG-B11A; ITG-B14A; ITG-B15A; DBM; U-VHELM; U-VEPID; JIF; DOI; PDF; Abstract; ITMPUB; DSPACE65 en_US
dc.description.abstract BACKGROUND: Taenia solium is a neglected zoonotic parasite. The performances of existing tools for the diagnosis of porcine cysticercosis need further assessment, and their shortcomings call for alternatives. The objective of this study was to evaluate the performance of tongue palpation and circulating antigen detection for the detection of porcine cysticercosis in naturally infected pigs of slaughter age compared to full carcass dissections (considered the gold standard). Additionally, alternative postmortem dissection procedures were investigated. A total of 68 rural pigs of slaughter age randomly selected in the Eastern Province of Zambia were dissected. Dissections were conducted on full carcasses (or half carcass in case cysticerci were already detected in the first half), including all the organs. Total cysticercus counts, location and stages were recorded and collected cysticerci were identified morphologically and molecularly. All sera were analysed with the B158/B60 antigen detecting ELISA (Ag-ELISA). RESULTS: Key findings were the high occurrence of T. solium infected pigs (56%) and the presence of T. solium cysticerci in the livers of 26% of infected animals. More than half of the infected carcasses contained viable cysticerci. Seven carcasses had T. hydatigena cysticerci (10%), out of which five carcasses were co-infected with T. hydatigena and T. solium; two carcasses (3%) had only T. hydatigena cysticerci. Compared to full carcass dissection, the specificity of the Ag-ELISA to detect infected carcasses was estimated at 67%, the sensitivity at 68%, increasing to 90% and 100% for the detection of carcasses with one or more viable cysticerci, and more than 10 viable cysts, respectively. Tongue palpation only detected 10% of the cases, half carcass dissection 84%. Selective dissection of the diaphragm, tongue and heart or masseters can be considered, with an estimated sensitivity of 71%, increasing to 86% in carcasses with more than 10 cysticerci. CONCLUSIONS: Depending on the aim of the diagnosis, a combination of Ag-ELISA and selective dissection, including investigating the presence of T. hydatigena, can be considered. Full carcass dissection should include the dissection of the liver, kidneys, spleen and lungs, and results should be interpreted carefully, as small cysticerci can easily be overlooked. en_US
dc.language English en_US
dc.relation.uri en_US
dc.subject Cysticercosis en_US
dc.subject Taenia solium en_US
dc.subject Helminthic diseases en_US
dc.subject Animal diseases en_US
dc.subject Pigs en_US
dc.subject Diagnosis en_US
dc.subject Detection en_US
dc.subject Dissection en_US
dc.subject Abattoirs en_US
dc.subject Zambia en_US
dc.subject Africa-Southern en_US
dc.title Re-visiting the detection of porcine cysticercosis based on full carcass dissections of naturally Taenia solium infected pigs en_US
dc.type Article en_US
dc.citation.issue 1 en_US
dc.citation.jtitle Parasites and Vectors en_US
dc.citation.volume 10 en_US
dc.citation.pages 572 en_US
dc.citation.abbreviation Parasit Vectors en_US

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