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Association of the endobiont doubled-stranded RNA virus LRV1 with treatment failure of human leishmaniasis caused by Leishmania braziliensis in Peru and Bolivia

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Show simple item record Adaui, V. en_US Lye, L. F. en_US Akopyants, N. S. en_US Zimic, M. en_US Llanos-Cuentas, A. en_US Garcia, L. en_US Maes, I. en_US De Doncker, S. en_US Dobson, D. E. en_US Arevalo, J. en_US Dujardin, J. C. en_US Beverley, S. M. en_US 2019-06-04T12:22:44Z 2019-06-04T12:22:44Z 2016 en_US
dc.identifier.issn 0022-1899 en_US
dc.identifier.doi en_US
dc.identifier.other en_US
dc.identifier.other 50 en_US
dc.identifier.other ITG-B7B; ITG-B8B; ITG-B11A; DBM; U-MOLPAR; JIF; DOI; PDF; Abstract; ITMPUB; DSPACE65 en_US
dc.description.abstract Cutaneous and mucosal leishmaniasis caused by Leishmania braziliensis in South America, is difficult to cure by chemotherapy (primarily pentavalent antimonials; Sb(V)). Treatment failure does not correlate well with resistance in vitro, and the factors responsible in patients are not well understood. Many isolates of L. braziliensis (>25%) contain a double-stranded RNA virus termed LRV1, also reported in L. guyanensis where association with increased pathology, metastasis, and parasite replication was found in murine models. Here we probed the relationship of LRV1 to drug treatment success and disease in L. braziliensis, in a group of 97 patients from Peru and Bolivia. In vitro cultures were established, parasites were typed as L. braziliensis, and the presence of LRV1 determined by RT-PCR followed by sequence analysis. LRV1 was associated significantly with increased risk of treatment failure (odds ratio=3.99; P=0.04). There was no significant association with intrinsic parasite Sb(V) resistance, suggesting that failures arise from LRV1-mediated effects on host metabolism and/or parasite survival. The association of LRV1 with clinical drug treatment failure could serve to guide more effective treatment of tegumentary disease caused by L. braziliensis. en_US
dc.language English en_US
dc.relation.uri en_US
dc.subject Protozoal diseases en_US
dc.subject Cutaneous en_US
dc.subject Mucosal en_US
dc.subject Leishmaniasis en_US
dc.subject Leishmania braziliensis en_US
dc.subject Vectors en_US
dc.subject Sandflies en_US
dc.subject Phlebotomus en_US
dc.subject Treatment failure en_US
dc.subject Isolation en_US
dc.subject RNA en_US
dc.subject Leishmaniavirus 1 (LRV1) en_US
dc.subject Reverse transcriptase inhibitors en_US
dc.subject Polymerase chain reaction en_US
dc.subject Sequence analysis en_US
dc.subject Metabolism en_US
dc.subject Parasitology en_US
dc.subject Survival en_US
dc.subject Peru en_US
dc.subject Bolivia en_US
dc.subject America-Latin en_US
dc.title Association of the endobiont doubled-stranded RNA virus LRV1 with treatment failure of human leishmaniasis caused by Leishmania braziliensis in Peru and Bolivia en_US
dc.type Article en_US
dc.citation.issue 1 en_US
dc.citation.jtitle Journal of Infectious Diseases en_US
dc.citation.volume 213 en_US
dc.citation.pages 112-121 en_US
dc.citation.abbreviation J Infect Dis en_US

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