Institute of Tropical Medicine Antwerp
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Characterization and pharmacological analysis of two adipokinetic hormone receptor variants of the tsetse fly, Glossina morsitans morsitans

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Show simple item record Caers, J. en_US Janssen, T. en_US Van Rompay, L. en_US Broeckx, V. en_US Van Den Abbeele, J. en_US Gade, G. en_US Schoofs, L. en_US Beets, I. en_US 2019-06-04T12:22:45Z 2019-06-04T12:22:45Z 2016 en_US
dc.identifier.issn 0965-1748 en_US
dc.identifier.doi en_US
dc.identifier.other This item is not available in the ITG library en_US
dc.identifier.other ITG-B5A; DBM- U-VPROT; JIF; DOI; Abstract; Not available; ITMPUB; DSPACE65 en_US
dc.description.abstract Adipokinetic hormones (AKH) are well known regulators of energy metabolism in insects. These neuropeptides are produced in the corpora cardiaca and perform their hormonal function by interacting with specific G protein-coupled receptors (GPCRs) at the cell membranes of target tissues, mainly the fat body. Here, we investigated the sequences, spatial and temporal distributions, and pharmacology of AKH neuropeptides and receptors in the tsetse fly, Glossina morsitans morsitans. The open reading frames of two splice variants of the Glomo-akh receptor (akhr) gene and of the AKH neuropeptide encoding genes, gmmhrth and gmmakh, were cloned. Both tsetse AKHR isoforms show strong sequence conservation when compared to other insect AKHRs. Glomo-AKH prepropeptides also have the typical architecture of AKH precursors. In an in vitro Ca2+ mobilization assay, Glomo-AKH neuropeptides activated each receptor isoform up to nanomolar concentrations. We identified structural features of tsetse AKH neuropeptides essential for receptor activation in vitro. Gene expression profiles suggest a function for AKH signaling in regulating Glossina energy metabolism, where AKH peptides are released from the corpora cardiaca and activate receptors mainly expressed in the fat body. This analysis of the ligand-receptor coupling, expression, and pharmacology of the two Glomo-AKHR variants facilitates further elucidation of the function of AKH in G. m. morsitans. en_US
dc.language English en_US
dc.relation.uri en_US
dc.subject Entomology en_US
dc.subject Tsetse flies en_US
dc.subject Glossina morsitans morsitans en_US
dc.subject Metabolism en_US
dc.subject Hormones en_US
dc.subject Neuropeptides en_US
dc.subject Sequence analysis en_US
dc.subject Pharmacology en_US
dc.subject AKH en_US
dc.subject Receptors en_US
dc.subject Gene expression en_US
dc.title Characterization and pharmacological analysis of two adipokinetic hormone receptor variants of the tsetse fly, Glossina morsitans morsitans en_US
dc.type Article en_US
dc.citation.jtitle Insect Biochemistry and Molecular Biology en_US
dc.citation.volume 70 en_US
dc.citation.pages 73-84 en_US
dc.citation.abbreviation Insect Biochem Mol Biol en_US

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