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First international external quality assessment scheme of nucleic acid amplification tests for the detection of Schistosoma and soil-transmitted helminths, including Strongyloides: a pilot study

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dc.contributor.author Cools, P. en_US
dc.contributor.author van Lieshout, L. en_US
dc.contributor.author Koelewijn, R. en_US
dc.contributor.author Addiss, D. en_US
dc.contributor.author Ajjampur, S. S. R. en_US
dc.contributor.author Ayana, M. en_US
dc.contributor.author Bradbury, R. S. en_US
dc.contributor.author Cantera, J. L. en_US
dc.contributor.author Dana, D. en_US
dc.contributor.author Fischer, K. en_US
dc.contributor.author Imtiaz, R. en_US
dc.contributor.author Kabagenyi, J. en_US
dc.contributor.author Lok, J. en_US
dc.contributor.author McCarthy, J. en_US
dc.contributor.author Mejia, R. en_US
dc.contributor.author Mekonnen, Z. en_US
dc.contributor.author Njenga, S. M. en_US
dc.contributor.author Othman, N. en_US
dc.contributor.author Shao, H. en_US
dc.contributor.author Traub, R. en_US
dc.contributor.author Van Esbroeck, M. en_US
dc.contributor.author Vercruysse, J. en_US
dc.contributor.author Vlaminck, J. en_US
dc.contributor.author Williams, S. A. en_US
dc.contributor.author Verweij, J. J. en_US
dc.contributor.author van Hellemond, J. J. en_US
dc.contributor.author Levecke, B. en_US
dc.date.accessioned 2020-08-25T09:54:14Z
dc.date.available 2020-08-25T09:54:14Z
dc.date.issued 2020 en_US
dc.identifier.issn 1935-2727 en_US
dc.identifier.doi http://dx.doi.org/10.1371/journal.pntd.0008231 en_US
dc.identifier.other ITG-C21A; DCS; U-CLCB; JIF; DOI; CPDF; ITMPUB; PMC; Abstract; DSPACE68 en_US
dc.identifier.uri http://hdl.handle.net/10390/10994
dc.description.abstract BACKGROUND: Nucleic acid amplification tests (NAATs) are increasingly being used as diagnostic tools for soil-transmitted helminths (STHs; Ascaris lumbricoides, Trichuris trichiura, Necator americanus, Ancylostoma duodenale and A. ceylanicum), Strongyloides stercoralis and Schistosoma in human stool. Currently, there is a large diversity of NAATs being applied, but an external quality assessment scheme (EQAS) for these diagnostics is lacking. An EQAS involves a blinded process where test results reported by a laboratory are compared to those reported by reference or expert laboratories, allowing for an objective assessment of the diagnostic performance of a laboratory. In the current study, we piloted an international EQAS for these helminths (i) to investigate the feasibility of designing and delivering an EQAS; (ii) to assess the diagnostic performance of laboratories; and (iii) to gain insights into the different NAAT protocols used. METHODS AND PRINCIPAL FINDINGS: A panel of twelve stool samples and eight DNA samples was validated by six expert laboratories for the presence of six helminths (Ascaris, Trichuris, N. americanus, Ancylostoma, Strongyloides and Schistosoma). Subsequently this panel was sent to 15 globally dispersed laboratories. We found a high degree of diversity among the different DNA extraction and NAAT protocols. Although most laboratories performed well, we could clearly identify the laboratories that were poorly performing. CONCLUSIONS/SIGNIFICANCE: We showed the technical feasibility of an international EQAS for the NAAT of STHs, Strongyloides and Schistosoma. In addition, we documented that there are clear benefits for participating laboratories, as they can confirm and/or improve the diagnostic performance of their NAATs. Further research should aim to identify factors that explain poor performance of NAATs. en_US
dc.language English en_US
dc.relation.uri http://www.ncbi.nlm.nih.gov/pubmed/32544158 en_US
dc.subject Helminthic diseases en_US
dc.subject Schistosomiasis en_US
dc.subject Soil-transmitted en_US
dc.subject Strongyloidiasis en_US
dc.subject Detection en_US
dc.subject Diagnostic tests en_US
dc.title First international external quality assessment scheme of nucleic acid amplification tests for the detection of Schistosoma and soil-transmitted helminths, including Strongyloides: a pilot study en_US
dc.type Article-E en_US
dc.citation.issue 6 en_US
dc.citation.jtitle PLoS Neglected Tropical Diseases en_US
dc.citation.volume 14 en_US
dc.citation.pages e0008231 en_US
dc.citation.abbreviation PLoS Negl Trop Dis en_US


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