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To pool or not to pool samples for sexually transmitted infections detection in men who have sex with men? An evaluation of a new pooling method using the GeneXpert instrument in West-Africa

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dc.contributor.author De Baetselier, I. en_US
dc.contributor.author Vuylsteke, B. en_US
dc.contributor.author Yaya, I. en_US
dc.contributor.author Dagnra, A. en_US
dc.contributor.author Diande, S. en_US
dc.contributor.author Mensah, E. en_US
dc.contributor.author Dah, E. en_US
dc.contributor.author Anoma, C. en_US
dc.contributor.author Kone, A. en_US
dc.contributor.author Faye-Kette, H. en_US
dc.contributor.author Yeo, A. en_US
dc.contributor.author Keita, B.D. en_US
dc.contributor.author Laurent, C. en_US
dc.contributor.author Crucitti, T. en_US
dc.date.accessioned 2020-08-25T09:54:16Z
dc.date.available 2020-08-25T09:54:16Z
dc.date.issued 2020 en_US
dc.identifier.issn 0148-5717 en_US
dc.identifier.doi http://dx.doi.org/10.1097/OLQ.0000000000001191 en_US
dc.identifier.other ITG-C1A; ITG-H2A; ITG-CLA; MULTI; DCS; U-ARLAB; DPH; U-HAP; JIF; DOI; CPDF; Abstract; ITMPUB; DSPACE68 en_US
dc.identifier.uri http://hdl.handle.net/10390/10997
dc.description.abstract BACKGROUND: Men who have sex with Men (MSM) using Pre-exposure prophylaxis (PrEP) are at risk for Sexually Transmitted Infections (STIs). Therefore, PrEP services should include regular screening for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) at urethra, anorectum and pharynx. However, financial and logistic challenges arise in low resource settings. We assessed a new STI sample pooling method using the GeneXpert instrument among MSM initiating PrEP in West-Africa. METHODS: Urine, anorectal and pharyngeal samples were pooled per individual for analysis. In case of an invalid result only (strategy one) or a positive result of the pool (strategy two), samples were analyzed individually to identify the infection's biological location. The results of two different pooling strategies were compared against the individual results obtained by a gold standard. RESULTS: We found a prevalence of 14.5% for chlamydia and 11.5% for gonorrhea, with a predominance of infections being extra-genital (77.6%). The majority of infections were asymptomatic (88.2%). The pooling strategy one, had a sensitivity, specificity and agreement for CT of 95.4%, 98.7% and 0.93, respectively; and 92.3%, 99.2% and 0.93 for pooling strategy two. For NG, these figures were 88.9%, 97.7% and 0.85 for strategy one, and 88.9%, 96.7% and 0.81 for strategy two. CONCLUSIONS: West-African MSM have a high prevalence of extra-genital and asymptomatic STIs. The GeneXpert method provides an opportunity to move from syndromic towards etiological STI diagnosis in low income countries, as the platform is available in African countries for tuberculosis testing. Pooling will reduce costs of triple site testing. en_US
dc.language English en_US
dc.relation.uri http://www.ncbi.nlm.nih.gov/pubmed/32355106 en_US
dc.subject Sexually transmitted diseases en_US
dc.subject STD en_US
dc.subject Detection en_US
dc.subject Genexpert en_US
dc.subject Homosexuals en_US
dc.subject Africa-West en_US
dc.title To pool or not to pool samples for sexually transmitted infections detection in men who have sex with men? An evaluation of a new pooling method using the GeneXpert instrument in West-Africa en_US
dc.type Article en_US
dc.citation.issue 8 en_US
dc.citation.jtitle Sexually Transmitted Diseases en_US
dc.citation.volume 47 en_US
dc.citation.pages 556-561 en_US
dc.citation.abbreviation Sex Transm Dis en_US


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