Institute of Tropical Medicine Antwerp
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Escherichia coli ST410 among humans and the environment in Southeast Asia

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Show simple item record Nadimpalli, M.L. en_US de Lauzanne, A. en_US Phe, T. en_US Borand, L. en_US Jacobs, J. en_US Fabre, L. en_US Naas, T. en_US Hello, S.L. en_US Stegger, M. en_US 2020-08-25T09:55:04Z 2020-08-25T09:55:04Z 2019 en_US
dc.identifier.issn 0924-8579 en_US
dc.identifier.doi en_US
dc.identifier.other en_US
dc.identifier.other 24 en_US
dc.identifier.other ITG-C5A; DCS; U-TLM; JIF; DOI; PDF; PMC; Abstract; ITMPUB; DSPACE68 en_US
dc.description.abstract OBJECTIVES: Escherichia coli ST410 (Ec-ST410) is an emerging, multidrug-resistant clone. Recent investigations of its global epidemiology and evolution have been based almost exclusively on isolates from Europe and North America. It is unclear whether Ec-ST410 in Southeast Asia belong to these same clones or represent regionally-disseminated variants. We characterized antibiotic resistance mechanisms among Southeast Asian-origin E. coli ST410 (SEA-Ec-ST410) and investigated whether they belonged to regional variants by contextualizing them within a global collection. METHODS: We identified 7 Ec-ST410 among a recent collection of expanded-spectrum cephalosporin-resistant E. coli recovered from 91 healthy women (stool) and 26 infected patients (blood and urine) living in Phnom Penh, Cambodia. We identified 9 additional Ec-ST410 genomes from Thailand (n=7) and Vietnam (n=2) through EnteroBase and PubMed searches. We characterized assembled genomes and created a SNP-based phylogenetic tree comparing these 16 SEA-Ec-ST410 to a previously published Ec-ST410 collection, primarily sourced from Europe (97/128) and North America (24/128). RESULTS: SEA-Ec-ST410 belonged to several distinct branches within previously described clonal clades. SEA-Ec-ST410 within the B3/H24Rx sub-lineage encoded blaCTX-M-55 genes (8/12) and F18:A-:B1 plasmid replicons (6/12), neither of which were detected among other Ec-ST410 belonging to this clade. Three of four SEA-Ec-ST410 within the B4/H24RxC sub-lineage lacked both the blaOXA-181 gene and an IncX3 plasmid replicon, which were harbored by 97% and 100% of all other Ec-ST410 in this sub-lineage (n=64), respectively. CONCLUSION: Ec-ST410 are present in Southeast Asia following multiple introductions. The unique pattern of antibiotic resistance elements harbored by SEA-Ec-ST410 suggests independent circulation in the region. en_US
dc.language English en_US
dc.relation.uri en_US
dc.subject Escherichia coli en_US
dc.subject Bacterial diseases en_US
dc.subject Asia-Southeast en_US
dc.title Escherichia coli ST410 among humans and the environment in Southeast Asia en_US
dc.type Article en_US
dc.citation.issue 2 en_US
dc.citation.jtitle International Journal of Antimicrobial Agents en_US
dc.citation.volume 54 en_US
dc.citation.pages 228-232 en_US
dc.citation.abbreviation Int J Antimicrob Agents en_US

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