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Molecular dipstick test for diagnosis of sleeping sickness

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dc.contributor.author Deborggraeve, S. en_US
dc.contributor.author Claes, F. en_US
dc.contributor.author Laurent, T. en_US
dc.contributor.author Mertens, P. en_US
dc.contributor.author Leclipteux, T. en_US
dc.contributor.author Dujardin, J. C. en_US
dc.contributor.author Herdewijn, P. en_US
dc.contributor.author Büscher, P. en_US
dc.date.accessioned 2007-12-06T14:33:22Z
dc.date.available 2007-12-06T14:33:22Z
dc.date.issued 2006 en_US
dc.identifier.issn 0095-1137 en_US
dc.identifier.doi http://dx.doi.org/10.1128/JCM.02594-05
dc.identifier.other ITG-P1A en_US
dc.identifier.other ITG-P2A en_US
dc.identifier.other ITG-P3B en_US
dc.identifier.other ITG-P6A en_US
dc.identifier.other ITG-PLA en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-SEROL en_US
dc.identifier.other U-PROTO en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.other FTC
dc.identifier.uri http://hdl.handle.net/10390/119
dc.description.abstract Human African trypanosomiasis (HAT) or sleeping sickness is a neglected disease that affects poor rural populations across sub-Saharan Africa. Confirmation of diagnosis is based on detection of parasites in either blood or lymph by microscopy. Here we present the development and the first-phase evaluation of a simple and rapid test (HAT-PCR-OC [human African trypanosomiasis-PCR-oligochromatography]) for detection of amplified Trypanosoma brucei DNA. PCR products are visualized on a dipstick through hybridization with a gold-conjugated probe (oligochromatography). Visualization is straightforward and takes only 5 min. Controls both for the PCR and for DNA migration are incorporated into the assay. The lower detection limit of the test is 5 fg of pure T. brucei DNA. One parasite in 180 microl of blood is still detectable. Sensitivity and specificity for T. brucei were calculated at 100% when tested on blood samples from 26 confirmed sleeping sickness patients, 18 negative controls (nonendemic region), and 50 negative control blood samples from an endemic region. HAT-PCR-OC is a promising new tool for diagnosis of sleeping sickness in laboratory settings, and the diagnostic format described here may have wider application for other infectious diseases. en_US
dc.language English en_US
dc.publisher American Society for Microbiology en_US
dc.subject Protozoal diseases en_US
dc.subject Trypanosomiasis, African en_US
dc.subject Trypanosoma brucei en_US
dc.subject Laboratory diagnosis en_US
dc.subject Rapid diagnostic tests en_US
dc.subject Dipstick en_US
dc.subject Sensitivity en_US
dc.subject Specificity en_US
dc.title Molecular dipstick test for diagnosis of sleeping sickness en_US
dc.type Article en_US
dc.citation.issue 8 en_US
dc.citation.jtitle Journal of Clinical Microbiology en_US
dc.citation.volume 44 en_US
dc.citation.pages 2884-2889 en_US
dc.publisher.place Washington en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/16891507
dc.citation.jabbreviation J Clin Microbiol en_US


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