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Pathogen genotyping in polyclonal infections: application of a fluorogenic polymerase-chain-reaction assay in malaria

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dc.contributor.author Decuypere, S. en_US
dc.contributor.author Elinck, E. en_US
dc.contributor.author Van Overmeir, C. en_US
dc.contributor.author Talisuna, A. O. en_US
dc.contributor.author D'Alessandro, U. en_US
dc.contributor.author Dujardin, J. C. en_US
dc.date.accessioned 2007-12-06T14:33:23Z
dc.date.available 2007-12-06T14:33:23Z
dc.date.issued 2003 en_US
dc.identifier.issn 0022-1899 en_US
dc.identifier.doi http://dx.doi.org/10.1086/378521
dc.identifier.other ITG-P1B en_US
dc.identifier.other ITG-P2B en_US
dc.identifier.other ITG-P3B en_US
dc.identifier.other ITG-P4B en_US
dc.identifier.other ITG-P5A en_US
dc.identifier.other ITG-PLA en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-PROTO en_US
dc.identifier.other U-MALAR en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other FTA en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/121
dc.description.abstract Pathogen genotyping of polyclonal infections is limited by 2 major drawbacks: (1). how to establish whether multiple mutations detected in 1 gene belong to the same clone and (2). how to evaluate the proportion of different genotypes in a given sample. For drug-resistance genotyping in Plasmodium falciparum malaria, we address these problems by using a fluorogenic assay that combines fluorescence-resonance energy transfer, between fluorophores present on a probe and a polymerase-chain-reaction primer, and a melt-curve analysis. We demonstrate that this tool allows a more accurate insight into the P. falciparum populations present in complex biological samples. en_US
dc.language English en_US
dc.publisher University of Chicago Press en_US
dc.subject Protozoal diseases en_US
dc.subject Malaria en_US
dc.subject Drug resistance en_US
dc.subject Genotyping en_US
dc.subject PCR en_US
dc.subject Assays en_US
dc.title Pathogen genotyping in polyclonal infections: application of a fluorogenic polymerase-chain-reaction assay in malaria en_US
dc.type Article en_US
dc.citation.issue 8 en_US
dc.citation.jtitle Journal of Infectious Diseases en_US
dc.citation.volume 188 en_US
dc.citation.pages 1245-1249 en_US
dc.publisher.place Chicago en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/14551896
dc.citation.jabbreviation J Infect Dis en_US


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