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A new PCR-ELISA for diagnosis of visceral leishmaniasis in blood of HIV-negative subjects

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dc.contributor.author De Doncker, S. en_US
dc.contributor.author Hutse, V. en_US
dc.contributor.author Abdellati, S. en_US
dc.contributor.author Rijal, S. en_US
dc.contributor.author Karki, B. M. S. en_US
dc.contributor.author Decuypere, S. en_US
dc.contributor.author Jacquet, D. en_US
dc.contributor.author Le Ray, D. en_US
dc.contributor.author Boelaert, M. en_US
dc.contributor.author Koirala, S. en_US
dc.contributor.author Dujardin, J. C. en_US
dc.date.accessioned 2007-12-06T14:33:24Z
dc.date.available 2007-12-06T14:33:24Z
dc.date.issued 2005 en_US
dc.identifier.issn 0035-9203 en_US
dc.identifier.doi http://dx.doi.org/10.1016/j.trstmh.2004.01.015
dc.identifier.other ITG-P1B en_US
dc.identifier.other ITG-P2B en_US
dc.identifier.other ITG-M3B en_US
dc.identifier.other ITG-P6B en_US
dc.identifier.other ITG-P7B en_US
dc.identifier.other ITG-X8P en_US
dc.identifier.other ITG-H9A en_US
dc.identifier.other ITG-PLA en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-PROTO en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-HIVSTD en_US
dc.identifier.other HEALTH en_US
dc.identifier.other U-EPID en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other MULTI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/124
dc.description.abstract The PCR-ELISA represents a promising advance for diagnosis of visceral leishmaniasis (VL) in blood samples. However, the method has been validated mostly with HIV-positive patients who are known to have high levels of parasitaemia. We developed a new PCR-ELISA assay for specific detection of Leishmania in patients' blood and validated it in Nepalese subjects with clinically suspected VL, almost all of whom were HIV-negative. For blood samples, PCR-ELISA was more sensitive (83.9%) than conventional PCR (73.2%), and demonstrated 100% and 87.2% specificity when using healthy controls who had never travelled to a VL-endemic area and controls from a VL-endemic area as references, respectively. We have demonstrated the ability of PCR-ELISA to detect parasites in blood of HIV-negative patients. The method could be used for epidemiological as well as clinical purposes, as it reduces the need for traumatic bone marrow sampling and risky spleen aspiration. en_US
dc.language English en_US
dc.publisher Elsevier
dc.subject Protozoal diseases en_US
dc.subject Leishmaniasis en_US
dc.subject Visceral en_US
dc.subject Molecular diagnostic techniques en_US
dc.subject Blood en_US
dc.subject PCR-ELISA en_US
dc.subject Sensitivity en_US
dc.subject Specificity en_US
dc.subject Nepal en_US
dc.subject Asia, South en_US
dc.title A new PCR-ELISA for diagnosis of visceral leishmaniasis in blood of HIV-negative subjects en_US
dc.type Article en_US
dc.citation.issue 1 en_US
dc.citation.jtitle Transactions of the Royal Society of Tropical Medicine and Hygiene en_US
dc.citation.volume 99 en_US
dc.citation.pages 25-31 en_US
dc.publisher.place Amsterdam
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/15550258
dc.identifier.url http://www.elsevier.com/locate/trstmh
dc.citation.jabbreviation Trans R Soc Trop Med Hyg en_US


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