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Clonal propagation and the fast generation of karyotype diversity; an in vitro Leishmania model

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dc.contributor.author Dujardin, J. C. en_US
dc.contributor.author De Doncker, S. en_US
dc.contributor.author Jacquet, D. en_US
dc.contributor.author Bañuls, A. L. en_US
dc.contributor.author Balavoine, M. en_US
dc.contributor.author Van Bockstaele, D. en_US
dc.contributor.author Tibayrenc, M. en_US
dc.contributor.author Arevalo, J. en_US
dc.contributor.author Le Ray, D. en_US
dc.date.accessioned 2007-12-06T14:33:25Z
dc.date.available 2007-12-06T14:33:25Z
dc.date.issued 2007 en_US
dc.identifier.issn 0031-1820 en_US
dc.identifier.doi http://dx.doi.org/10.1017/S0031182006001156
dc.identifier.other ITG-P1A en_US
dc.identifier.other ITG-P2B en_US
dc.identifier.other ITG-I3B en_US
dc.identifier.other ITG-XLP en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-PROTO en_US
dc.identifier.other INTER en_US
dc.identifier.other U-TTP en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other FTA en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/130
dc.description.abstract In the present work we studied the karyotype stability during long-term in vitro maintenance in 3 cloned strains of Leishmania (Viannia) peruviana, Leishmania (Viannia) braziliensis and a hybrid between both species. Only the L. (V.) peruviana strain showed an unstable karyotype, even after subcloning. Four chromosomes were studied in detail, each of them characterized by homologous chromosomes of different size (heteromorphy). Variations in chromosome patterns during in vitro maintenance were rapid and discrete, involving loss of heteromorphy or appearance of additional chromosome size variants. The resulting pattern was not the same according to experimental conditions (subinoculation rate or incubation temperature), and interestingly, this was associated with differences in growth behaviour of the respective parasites. No change in total ploidy of the cells was observed by flow cytometry. We discuss several mechanisms that might account for this variation of chromosome patterns, but we favour the occurrence of aneuploidy, caused by aberrant chromosome segregation during mitosis. Our results provide insight into the generation of karyotype diversity in natural conditions and highlight the relativity of the clone concept in parasitology. en_US
dc.language English en_US
dc.publisher Cambridge University Press en_US
dc.subject Protozoology en_US
dc.subject Evolutionary genetics en_US
dc.subject Leishmania peruviana en_US
dc.subject Leishmania braziliensis en_US
dc.subject Clones en_US
dc.subject Karyotypes en_US
dc.subject Polymorphism en_US
dc.subject Chromosomal size variation en_US
dc.subject Ploidy en_US
dc.title Clonal propagation and the fast generation of karyotype diversity; an in vitro Leishmania model en_US
dc.type Article en_US
dc.citation.issue 1 en_US
dc.citation.jtitle Parasitology en_US
dc.citation.volume 134 en_US
dc.citation.pages 33-39 en_US
dc.publisher.place Cambridge en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/16978449
dc.citation.jabbreviation Parasitology en_US


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