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American tegumentary leishmaniasis: direct species identification of Leishmania in non-invasive clinical samples

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dc.contributor.author Garcia, A. L. en_US
dc.contributor.author Parrado, R. en_US
dc.contributor.author De Doncker, S. en_US
dc.contributor.author Bermudez, H. en_US
dc.contributor.author Dujardin, J. C. en_US
dc.date.accessioned 2007-12-06T14:33:31Z
dc.date.available 2007-12-06T14:33:31Z
dc.date.issued 2007 en_US
dc.identifier.issn 0035-9203 en_US
dc.identifier.doi http://dx.doi.org/10.1016/j.trstmh.2006.06.009
dc.identifier.other ITG-P1B en_US
dc.identifier.other ITG-P3B en_US
dc.identifier.other ITG-PLA en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-PROTO en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/150
dc.description.abstract Species identification is highly relevant for improved prognosis and adequate treatment of American tegumentary leishmaniasis (ATL). PCR-based methods are available for this purpose but should be simplified to improve accessibility. As a first step in this process, this paper describes a simplified protocol for collection of clinical samples. Using samples from 44 Bolivian patients with confirmed ATL, we demonstrated that hsp70 PCR-RFLP on skin scrapings collected with a tooth pick allowed identification of the parasite species with a sensitivity of 95% and specificity of 100%. Our method should greatly facilitate individual patient management and epidemiological surveillance of ATL. en_US
dc.language English en_US
dc.publisher Elsevier
dc.subject Protozoal diseases en_US
dc.subject Leishmaniasis en_US
dc.subject Tegumentary en_US
dc.subject Cutaneous en_US
dc.subject Species en_US
dc.subject Leishmania braziliensis en_US
dc.subject Leishmania lainsoni en_US
dc.subject Identification en_US
dc.subject Molecular diagnostic techniques en_US
dc.subject PCR en_US
dc.subject Simplified en_US
dc.subject Sensitivity en_US
dc.subject Specificity en_US
dc.subject Bolivia en_US
dc.subject America, Latin en_US
dc.title American tegumentary leishmaniasis: direct species identification of Leishmania in non-invasive clinical samples en_US
dc.type Article en_US
dc.citation.issue 4 en_US
dc.citation.jtitle Transactions of the Royal Society of Tropical Medicine and Hygiene en_US
dc.citation.volume 101 en_US
dc.citation.pages 368-371 en_US
dc.publisher.place Amsterdam
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/17011005
dc.identifier.url http://www.elsevier.com/locate/trstmh
dc.citation.jabbreviation Trans R Soc Trop Med Hyg en_US


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