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Comparative evaluation of freeze-dried and liquid antigens in the direct agglutination test for serodiagnosis of visceral leishmaniasis (ITMA-DAT/VL)

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dc.contributor.author Jacquet, D. en_US
dc.contributor.author Boelaert, M. en_US
dc.contributor.author Seaman, J. en_US
dc.contributor.author Rijal, S. en_US
dc.contributor.author Sundar, S. en_US
dc.contributor.author Menten, J. en_US
dc.contributor.author Magnus, E. en_US
dc.date.accessioned 2007-12-06T14:45:12Z
dc.date.available 2007-12-06T14:45:12Z
dc.date.issued 2006 en_US
dc.identifier.issn 1360-2276 en_US
dc.identifier.doi http://dx.doi.org/10.1111/j.1365-3156.2006.01743.x
dc.identifier.other ITG-I1B en_US
dc.identifier.other ITG-H2A en_US
dc.identifier.other ITG-I6A en_US
dc.identifier.other ITG-ILB en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-SEROL en_US
dc.identifier.other HEALTH en_US
dc.identifier.other U-EPID en_US
dc.identifier.other INTER en_US
dc.identifier.other U-CTU en_US
dc.identifier.other U-TTP en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other MULTI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.other FTB en_US
dc.identifier.uri http://hdl.handle.net/10390/1626
dc.description The definitive version is available at www3.interscience.wiley.com
dc.description.abstract OBJECTIVE: The direct agglutination test (DAT) for visceral leishmaniasis (VL) with liquid (LQ) antigen is known to be only moderately reproducible because of inter-observer and batch-to-batch variability as well as its sensitivity to temperature and shaking during transport. We evaluated a DAT with freeze-dried (FD) antigen and compared it with the LQ antigen version. METHODS: Blood samples of clinical VL suspects and healthy endemic controls were collected in Sudan, Nepal and India. Both test versions were performed in duplicate in the respective countries and in the reference laboratory. Interbatch variability and stability tests were conducted and agreement was examined within and between centres on a dichotomic scale by Cohen's kappa as well as on a continuous scale through Bland-Altman plots. RESULTS: The FD antigen remains fully active even after storage at 45 degrees C for 24 months. Using a cut-off titre of 1:6400, the agreement between the FD and the LQ formats was excellent. CONCLUSION: The major advantages of FD antigen are its better stability at higher temperatures and its longer shelf life, which make it much more suitable than the LQ version for use in the field. en_US
dc.language English en_US
dc.publisher Blackwell Publishing
dc.subject Protozoal diseases en_US
dc.subject Leishmaniasis en_US
dc.subject Visceral en_US
dc.subject Serodiagnosis en_US
dc.subject DAT en_US
dc.subject Antigens en_US
dc.subject Freeze-dried en_US
dc.title Comparative evaluation of freeze-dried and liquid antigens in the direct agglutination test for serodiagnosis of visceral leishmaniasis (ITMA-DAT/VL) en_US
dc.type Article en_US
dc.citation.issue 12 en_US
dc.citation.jtitle Tropical Medicine and International Health en_US
dc.citation.volume 11 en_US
dc.citation.pages 1777-1784 en_US
dc.publisher.place Oxford
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/17176341
dc.citation.jabbreviation Trop Med Int Health en_US


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