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SYBR Green-based quantitation of human T-lymphotropic virus type 1 proviral load in Peruvian patients with neurological disease and asymptomatic carriers; influence of clinical status, sex, and familial relatedness

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dc.contributor.author Adaui, V. en_US
dc.contributor.author Verdonck, K. en_US
dc.contributor.author Best, I. en_US
dc.contributor.author Gonzalez, E. en_US
dc.contributor.author Tipismana, M. en_US
dc.contributor.author Arévalo, J. en_US
dc.contributor.author Vanham, G. en_US
dc.contributor.author Campos, M. en_US
dc.contributor.author Zimic, M. en_US
dc.contributor.author Gotuzzo, E. en_US
dc.date.accessioned 2007-12-06T14:46:19Z
dc.date.available 2007-12-06T14:46:19Z
dc.date.issued 2006 en_US
dc.identifier.issn 1355-0284 en_US
dc.identifier.doi http://dx.doi.org/10.1080/13550280601039634
dc.identifier.other ITG-M2A en_US
dc.identifier.other ITG-M7A en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-VIROL en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/1650
dc.description.abstract To evaluate the human T-lymphotropic virus type 1 (HTLV-1) proviral DNA load in patients with HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and asymptomatic HTLV-1 carriers, a SYBR Green-based real-time quantitative polymerase chain reaction (qPCR) assay was developed. HTLV-1 proviral DNA in peripheral blood mononuclear cells (PBMCs) was quantified using primers targeting the pX region and the HTLV-1 copy number normalized to the amount of ERV-3 (Endogenous Retrovirus 3) cellular DNA. Thirty-three asymptomatic HTLV-1 carriers (ACs) and 39 patients with HAM/TSP were enrolled. Some participants were relatives of HAM/TSP cases (16 ACs and 7 patients with HAM/TSP). On multiple linear regression analysis, the authors found a significant association between clinical status and HTLV-1 proviral load (P < .01), but only among women. ACs showed a median proviral load of 561 copies per 104 PBMCs (interquartile range: 251-1623). In HAM/TSP patients, the median proviral load was 1783 (1385-2914). ACs related to HAM/TSP patients presented a relatively high proviral load (median 1152); however, the association between relatedness to a HAM/TSP patient and proviral load was not significant (P = .1). In HAM/TSP patients, no association was found between proviral load and disease duration, progression or severity. The fact that the effect of HAM/TSP upon the HTLV-1 proviral load differed between sexes and the finding of a high proviral load among asymptomatic relatives of HAM/TSP patients suggest that not yet identified genetic or environmental factors influence the pathogenesis of HTLV-1 infection. en_US
dc.language English en_US
dc.subject Viral diseases en_US
dc.subject HTLV-1 en_US
dc.subject Proviral load en_US
dc.subject Quantitation en_US
dc.subject Tropical spastic paraparesis en_US
dc.subject Carrier state en_US
dc.subject Risk factors en_US
dc.subject Peru en_US
dc.subject America, Latin en_US
dc.title SYBR Green-based quantitation of human T-lymphotropic virus type 1 proviral load in Peruvian patients with neurological disease and asymptomatic carriers; influence of clinical status, sex, and familial relatedness en_US
dc.type Article en_US
dc.citation.issue 6 en_US
dc.citation.jtitle Journal of Neurovirology en_US
dc.citation.volume 12 en_US
dc.citation.pages 456-465 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/17162661
dc.citation.jabbreviation J Neurovirol en_US


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