Institute of Tropical Medicine Antwerp
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Replicative fitness of historical and recent HIV-1 isolates suggests HIV-1 attenuation over time

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dc.contributor.author Ariën, K. K. en_US
dc.contributor.author Troyer, R. M. en_US
dc.contributor.author Gali, Y. en_US
dc.contributor.author Colebunders, R. en_US
dc.contributor.author Arts, E. J. en_US
dc.contributor.author Vanham, G. en_US
dc.date.accessioned 2007-12-06T14:46:23Z
dc.date.available 2007-12-06T14:46:23Z
dc.date.issued 2005 en_US
dc.identifier.issn 0269-9370 en_US
dc.identifier.other ITG-M1A en_US
dc.identifier.other ITG-M3B en_US
dc.identifier.other ITG-C4A en_US
dc.identifier.other ITG-MLA en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-VIROL en_US
dc.identifier.other CLINIC en_US
dc.identifier.other U-HIVCLI en_US
dc.identifier.other JIF en_US
dc.identifier.other MULTI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/1660
dc.description Not the final published version
dc.description.abstract BACKGROUND: Changes in virulence during an epidemic are common among pathogens, but still unexplored in the case of HIV-1. Here we used primary human cells to study the replicative fitness of primary HIV-1 isolates from untreated patients, comparing historical (1986-1989) and recent samples (2002-2003). METHODS: Head-to-head dual virus infection/competition assays were performed in both peripheral blood mononuclear cells and human dendritic cell/T-cell co-cultures with pairs of 12 carefully matched historical and recent HIV-1 isolates from untreated patients. Sensitivity to inhibition by lamivudine (3TC) and TAK-779 of historical and recent R5 HIV-1 isolates was measured in a subset of samples. RESULTS: Overall, the historical HIV-1 out-competed the recent HIV-1 isolates in 176 of 238 competitions and in 9 of 12 competitions carefully matched for CD4 cell count. The mean relative replicative fitness (W) of all historical HIV-1 strains was significantly greater than that of recent HIV-1 isolates (W(1986-1989) = 1.395 and W(2002-2003) = 0.545, P < 0.001 (t test)). The more fit viruses (mean W > 1) from 1986-1989 appeared less sensitive to TAK-779 and 3TC than did the less fit (mean W < 1) 2002-2003 viruses. CONCLUSIONS: These findings suggest that HIV-1 replicative fitness may have decreased in the human population since the start of the pandemic. This 'attenuation' could be the consequence of serial bottlenecks during transmission and result in adaptation of HIV-1 to the human host. en_US
dc.language English en_US
dc.publisher Lippincott, Williams & Wilkins
dc.subject Virology en_US
dc.subject HIV-1 en_US
dc.subject Transmission en_US
dc.subject Virus replication en_US
dc.subject Viral fitness en_US
dc.subject Trends en_US
dc.subject Attenuation en_US
dc.subject Adaptation en_US
dc.title Replicative fitness of historical and recent HIV-1 isolates suggests HIV-1 attenuation over time en_US
dc.type Article en_US
dc.citation.issue 15 en_US
dc.citation.jtitle AIDS en_US
dc.citation.volume 19 en_US
dc.citation.pages 1555-1564 en_US
dc.publisher.place Philadelphia
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/16184024
dc.citation.jabbreviation AIDS en_US


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