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Systematic restaining of sputum smears for quality control is useful in Burundi

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Show simple item record Buzingo, T. en_US Sanders, M. en_US Masabo, J. P. en_US Nyandwi, S. en_US Van Deun, A. en_US 2007-12-06T14:46:36Z 2007-12-06T14:46:36Z 2003 en_US
dc.identifier.issn 1027-3719 en_US
dc.identifier.other ITG-MLA en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-MYCOB en_US
dc.identifier.other JIF en_US
dc.identifier.other ABSTRACT en_US
dc.description.abstract SETTING: Routine tuberculosis control services in Burundi. OBJECTIVE: To determine whether systematic restaining of sputum smears for acid-fast bacilli (AFB) prior to rechecking quality assessment is necessary. DESIGN: Blinded rechecking of peripheral routine smears, including a second control of discordants, before and after restaining. RESULTS: Without restaining, 10/825 (1.2%) negative, and 59/189 (31.2%) positive results were declared false. After restaining, there were 34 (4.1%) false negatives and 13 (6.9%) false positives, both highly significant changes. Before restaining, quantification of positive smears was usually considered too high, while after restaining 41 out of 42 positives were found to have too low readings. CONCLUSIONS: Despite mild climatic conditions in Burundi, restaining all slides before rechecking revealed an unrecognised, widespread problem of false negatives, rather than false positives. This indicated the need for critical re-appraisal of the standard procedure using cold staining, rather than re-training, as would have been inferred from results without restaining. Systematic restaining of all slides prior to rechecking may be more widely needed in National Tuberculosis Programmes to cover all possible serious causes of error. Cold staining should be avoided in field programmes since its performance is easily affected by frequently encountered adverse factors. en_US
dc.language English en_US
dc.publisher International Union Against Tuberculosis And Lung Disease
dc.subject Bacterial diseases en_US
dc.subject Tuberculosis en_US
dc.subject Laboratory diagnosis en_US
dc.subject Sputum smear microscopy en_US
dc.subject Staining en_US
dc.subject Quality control en_US
dc.subject Burundi en_US
dc.title Systematic restaining of sputum smears for quality control is useful in Burundi en_US
dc.type Article en_US
dc.citation.jtitle International Journal of Tuberculosis and Lung Disease en_US
dc.citation.volume 7 en_US
dc.citation.pages 439-444 en_US Paris
dc.citation.jabbreviation Int J Tuberc Lung Dis en_US

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