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Study of HIV type 1 gag/env variability in The Gambia, using a multiplex DNA polymerase chain reaction

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dc.contributor.author Cham, F. en_US
dc.contributor.author Heyndrickx, L. en_US
dc.contributor.author Janssens, W. en_US
dc.contributor.author Van der Auwera, G. en_US
dc.contributor.author Vereecken, K. en_US
dc.contributor.author De Houwer, K. en_US
dc.contributor.author Coppens, S. en_US
dc.contributor.author Whittle, H. en_US
dc.contributor.author van der Groen, G. en_US
dc.date.accessioned 2007-12-06T14:46:38Z
dc.date.available 2007-12-06T14:46:38Z
dc.date.issued 2000 en_US
dc.identifier.issn 0889-2229 en_US
dc.identifier.doi http://dx.doi.org/10.1089/08892220050195874
dc.identifier.other ITG-M1B en_US
dc.identifier.other ITG-M2A en_US
dc.identifier.other ITG-M3A en_US
dc.identifier.other ITG-M4A en_US
dc.identifier.other ITG-M5B en_US
dc.identifier.other ITG-M6B en_US
dc.identifier.other ITG-M7B en_US
dc.identifier.other ITG-MLA en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-VIROL en_US
dc.identifier.other JIF en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.other FTA
dc.identifier.uri http://hdl.handle.net/10390/1702
dc.description.abstract A multiplex DNA PCR assay was developed for the simultaneous first-round amplification of HIV-1 gag and env fragments for the heteroduplex mobility assay (HMA). This assay was compared with the conventional amplification assay, using DNA extracted from PBMC samples from 30 HIV-1-seropositive individuals from The Gambia, who were enrolled between 1992 and 1997. From 27 of 30 (90%) samples both gag and env HMA fragments were amplified simultaneously. In one sample only the gag HMA fragment could be amplified by multiplex DNA PCR, and in two samples amplification was negative for both gag and env HMA in multiplex as well as the mono-DNA PCR. Of the 28 Gambian isolates subtyped by gag/env HMA or by sequencing and phylogenetic analysis, the majority (19 of 28; 68%) were intersubtype recombinant. Fifteen of 28 (53%) samples were circulating recombinant form (CRF) CRF02.AG variants. Two isolates clustering with the previously documented Gambian isolate GM4 (previously described as an env GC recombinant) are classified as gag A/env J recombinants. en_US
dc.language English en_US
dc.subject Virology en_US
dc.subject HIV-1 env en_US
dc.subject Genetics en_US
dc.subject Variability en_US
dc.subject Gambia en_US
dc.subject Africa, West en_US
dc.title Study of HIV type 1 gag/env variability in The Gambia, using a multiplex DNA polymerase chain reaction en_US
dc.type Article en_US
dc.citation.issue 17 en_US
dc.citation.jtitle AIDS Research and Human Retroviruses en_US
dc.citation.volume 17 en_US
dc.citation.pages 1915-1919 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/11118077
dc.citation.jabbreviation AIDS Res Hum Retroviruses en_US


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