Institute of Tropical Medicine Antwerp
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Evaluation of rifampicin and isoniazid susceptibility testing of Mycobacterium tuberculosis by a mycobacteriophage D29-based assay

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dc.contributor.author Chauca, J. A. en_US
dc.contributor.author Palomino, J. C. en_US
dc.contributor.author Guerra, H. en_US
dc.date.accessioned 2007-12-06T14:46:39Z
dc.date.available 2007-12-06T14:46:39Z
dc.date.issued 2007 en_US
dc.identifier.issn 0022-2615 en_US
dc.identifier.doi http://dx.doi.org/10.1099/jmm.0.46622-0
dc.identifier.other ITG-M2A en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-MYCOB en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/1705
dc.description.abstract Conventional methods for determining drug susceptibility of Mycobacterium tuberculosis require several weeks to obtain results, limiting their usefulness; automated methods and those based on molecular biology techniques have been able to reduce the turnaround time, but their high cost and need for sophisticated equipment restrict their use in developing countries. The goal of the present study was to evaluate the diagnostic accuracy of a rapid (3-4 days) low-cost test based on the use of mycobacteriophage D29 to determine the susceptibility of strains of M. tuberculosis to rifampicin (RIF) and isoniazid (INH). Results obtained show that susceptibility testing for RIF has a high diagnostic accuracy as compared to the standard indirect proportion method on Löwenstein-Jensen medium (sensitivity 100% and specificity 98%). Given the association between the resistance to RIF and INH, which define multidrug resistance (MDR), this test seems suitable for rapid detection of MDR tuberculosis strains (kappa=0.978). Susceptibility testing for INH using mycobacteriophage D29 had a good but lower diagnostic accuracy as compared to the standard method (sensitivity 80.4% and specificity 80.8%); the test would then be of limited usefulness in the management of tuberculosis patients. Further studies to determine the relationship of mycobacteriophage D29 tests to in vivo correlates of sensitivity to specific antituberculosis drugs are warranted. en_US
dc.language English en_US
dc.subject Bacterial diseases en_US
dc.subject Tuberculosis en_US
dc.subject Drug therapy en_US
dc.subject Isoniazid en_US
dc.subject Rifampicin en_US
dc.subject Drug sensitivity testing en_US
dc.subject Rapid diagnostic tests en_US
dc.subject D29 en_US
dc.subject Evaluation en_US
dc.subject Sensitivity en_US
dc.subject Specificity en_US
dc.subject Affordability en_US
dc.title Evaluation of rifampicin and isoniazid susceptibility testing of Mycobacterium tuberculosis by a mycobacteriophage D29-based assay en_US
dc.type Article en_US
dc.citation.issue 3 en_US
dc.citation.jtitle Journal of Medical Microbiology en_US
dc.citation.volume 56 en_US
dc.citation.pages 360-364 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/17314367
dc.citation.jabbreviation J Med Microbiol en_US


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