Institute of Tropical Medicine Antwerp
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Characterization of an unusual mycobacterium: a possible missing link between Mycobacterium marinum and Mycobacterium ulcerans

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Show simple item record Chemlal, K. en_US Huys, G. en_US Laval, F. en_US Vincent, V. en_US Savage, C. en_US Gutierrez, C. en_US Laneelle, M. A. en_US Swings, J. en_US Meyers, W. M. en_US Daffe, M. en_US Portaels, F. en_US 2007-12-06T14:46:40Z 2007-12-06T14:46:40Z 2002 en_US
dc.identifier.issn 0095-1137 en_US
dc.identifier.other ITG-M1B en_US
dc.identifier.other ITG-MLA en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-MYCOB en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.description.abstract In an attempt to characterize an unusual mycobacterial isolate from a 44-year-old patient living in France, we applied phenotypic characterizations and various previously described molecular methods for the taxonomic classification of mycobacteria. The results of the investigations were compared to those obtained in a previous study with a set of temporally and geographically diverse Mycobacterium ulcerans (n = 29) and Mycobacterium marinum (n = 29) isolates (K. Chemlal, G. Huys, P.-A. Fonteyne, V. Vincent, A. G. Lopez, L. Rigouts, J. Swings, W. M. Meyers, and F. Portaels, J. Clin. Microbiol. 39:3272-3278, 2001). The isolate, designated ITM 00-1026 (IPP 2000-372), is closely related to M. marinum according to its phenotypic properties, lipid pattern, and partial 16S rRNA sequence. Moreover, fingerprinting by amplified fragment length polymorphism (AFLP) analysis unequivocally classified this strain as a member of the species M. marinum, although it lacked two species-specific AFLP marker bands. However, PCR and restriction fragment length polymorphism analysis based on M. ulcerans-specific insertion sequence IS2404 showed the presence of this element in a low copy number in isolate ITM 00-1026. In conclusion, the designation of this isolate as a transitional species further supports the recent claim by Stinear et al. (T. Stinear, G. Jenkin, P. D. Johnson, and J. K. Davies, J. Bacteriol. 182:6322-6330, 2000) that M. ulcerans represents a relatively recent phylogenetic derivative of M. marinum resulting from the systematic acquisition of foreign DNA fragments. en_US
dc.language English en_US
dc.publisher American Society for Microbiology en_US
dc.subject Bacteriology en_US
dc.subject Mycobacteriology en_US
dc.subject Characterization en_US
dc.subject Species en_US
dc.subject Molecular biology en_US
dc.subject PCR en_US
dc.subject Genotyping en_US
dc.subject Phenotypes en_US
dc.subject Lipid analysis en_US
dc.subject RFLP en_US
dc.subject Mycobacterium ulcerans en_US
dc.title Characterization of an unusual mycobacterium: a possible missing link between Mycobacterium marinum and Mycobacterium ulcerans en_US
dc.type Article en_US
dc.citation.issue 7 en_US
dc.citation.jtitle Journal of Clinical Microbiology en_US
dc.citation.volume 40 en_US
dc.citation.pages 2370-2380 en_US Washington en_US
dc.citation.jabbreviation J Clin Microbiol en_US

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