Institute of Tropical Medicine Antwerp
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Performance evaluation of the two protease sequencing primers of the Trugene HIV-1 genotyping kit

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dc.contributor.author Garc¡a Ribas, S. en_US
dc.contributor.author Heyndrickx, L. en_US
dc.contributor.author Ondoa, P. en_US
dc.contributor.author Fransen, K. en_US
dc.date.accessioned 2007-12-06T14:47:00Z
dc.date.available 2007-12-06T14:47:00Z
dc.date.issued 2006 en_US
dc.identifier.issn 0166-0934 en_US
dc.identifier.doi http://dx.doi.org/10.1016/j.viromet.2006.05.010
dc.identifier.other ITG-M1B en_US
dc.identifier.other ITG-M2A en_US
dc.identifier.other ITG-M3A en_US
dc.identifier.other ITG-MLA en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-IMMUN en_US
dc.identifier.other U-VIROL en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/1765
dc.description.abstract This article describes the performance of the two protease sequencing modules available in the Trugene HIV-1 genotyping Kit on a sample population with a high prevalence of HIV-1 non-B subtypes (n=110). The relevance of the algorithm recommended by the kit was also evaluated. The results indicated a high sequencing failure rate of the PR module (34%). Forty-five percent of the failed sequences derived from non-B subtype viruses. Furthermore, no PR sequence could be obtained from any of the HIV-1 subtype A and C infected samples that were tested. In contrast, a sequence could be obtained from the entire panel using the P2 module. The data indicated that the high rate of sequencing failures of the PR module was related to both the HIV-1 non-B subtypes as well as lower levels of RNA viral load. In six out of the 73 samples for which both protease modules were successful, discrepancies between the two protease sequences were observed, which led to discordant resistance reports in two cases. The data highlight the problems and the clinical implications that may occur during resistance genotyping of clinical samples with a high prevalence of HIV-1 non-B subtypes. en_US
dc.language English en_US
dc.subject Virology en_US
dc.subject HIV-1 en_US
dc.subject Genotyping en_US
dc.subject Subtypes en_US
dc.subject Protease en_US
dc.subject Primers en_US
dc.subject Trugene en_US
dc.subject Evaluation studies en_US
dc.title Performance evaluation of the two protease sequencing primers of the Trugene HIV-1 genotyping kit en_US
dc.type Article en_US
dc.citation.issue 2 en_US
dc.citation.jtitle Journal of Virological Methods en_US
dc.citation.volume 135 en_US
dc.citation.pages 137-142 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/16777242
dc.citation.jabbreviation J Virol Methods en_US


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