Institute of Tropical Medicine Antwerp
Foundation of Public Utility

Evaluation of mycobacteria growth indicator tube for direct and indirect drug susceptibility testing of Mycobacterium tuberculosis from respiratory specimens in a Siberian prison hospital

DSpace/Manakin Repository

Show simple item record

dc.contributor.author Goloubeva, V. en_US
dc.contributor.author Lecocq, M. en_US
dc.contributor.author Lassowsky, P. en_US
dc.contributor.author Matthys, F. en_US
dc.contributor.author Portaels, F. en_US
dc.contributor.author Bastian, I. en_US
dc.date.accessioned 2007-12-06T14:47:04Z
dc.date.available 2007-12-06T14:47:04Z
dc.date.issued 2001 en_US
dc.identifier.issn 0095-1137 en_US
dc.identifier.doi http://dx.doi.org/10.1128/JCM.39.4.1501.2001
dc.identifier.other ITG-M5A en_US
dc.identifier.other ITG-MLA en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-MYCOB en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/1777
dc.description.abstract The manual Mycobacteria Growth Indicator Tube (MGIT) method was evaluated for performing direct and indirect drug susceptibility testing (DST) of Mycobacterium tuberculosis for isoniazid and rifampin on 101 strongly smear-positive sputum specimens in a Siberian prison hospital. Using the indirect method of proportion (MOP) as the "gold standard," the accuracies of isoniazid and rifampin susceptibility testing by the direct MGIT system were 97.0 and 94.1%, respectively. The accuracy of the indirect MGIT system was 98.0% for both drugs. The turnaround times from specimen processing to reporting of the DST results ranged between 4 and 23 (mean, 9.2) days by the direct MGIT method, 9 and 30 (mean, 15.3) days by the indirect MGIT method, and 26 and 101 (mean, 59.6) days by the indirect MOP. MGIT appears to be a reliable, rapid, and convenient method for performing direct and indirect DSTs in low-resource settings, but further studies are required to refine the direct DST protocol. Cost is the only factor prohibiting widespread implementation of MGIT. en_US
dc.language English en_US
dc.publisher American Society for Microbiology en_US
dc.subject Bacterial diseases en_US
dc.subject Tuberculosis en_US
dc.subject Mycobacterium tuberculosis en_US
dc.subject Drug resistance en_US
dc.subject Isoniazid en_US
dc.subject Rifampin en_US
dc.subject Drug sensitivity testing en_US
dc.subject Sputum smear microscopy en_US
dc.subject Russia en_US
dc.subject Asia en_US
dc.title Evaluation of mycobacteria growth indicator tube for direct and indirect drug susceptibility testing of Mycobacterium tuberculosis from respiratory specimens in a Siberian prison hospital en_US
dc.type Article en_US
dc.citation.issue 4 en_US
dc.citation.jtitle Journal of Clinical Microbiology en_US
dc.citation.volume 39 en_US
dc.citation.pages 1501 en_US
dc.publisher.place Washington en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/11283077
dc.citation.jabbreviation J Clin Microbiol en_US


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record