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HIV-1 subtype H near-full length genome reference strains and analysis of subtype-H-containing inter-subtype recombinants

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dc.contributor.author Janssens, W. en_US
dc.contributor.author Laukkanen, T. en_US
dc.contributor.author Salminen, M. O. en_US
dc.contributor.author Carr, J. K. en_US
dc.contributor.author Van der Auwera, G. en_US
dc.contributor.author Heyndrickx, L. en_US
dc.contributor.author van der Groen, G. en_US
dc.contributor.author McCutchan, F. E. en_US
dc.date.accessioned 2007-12-06T14:47:11Z
dc.date.available 2007-12-06T14:47:11Z
dc.date.issued 2000 en_US
dc.identifier.issn 0269-9370 en_US
dc.identifier.other ITG-M1A en_US
dc.identifier.other ITG-M5A en_US
dc.identifier.other ITG-M6A en_US
dc.identifier.other ITG-M7A en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-VIROL en_US
dc.identifier.other JIF en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/1795
dc.description Not the final published version en_US
dc.description.abstract OBJECTIVE: To characterize near-full-length genomes of two HIV-1 subtype H strains. To extend sequence data to include full env and gag, and analyse and redefine, previously documented subtype H strains. DESIGN: Near-full-length genomes of HIV-1 env subtype H strains VI991 and VI997 were amplified, cloned, sequenced, phylogenetically analysed and compared with a panel of 23 HIV-1 group M reference isolates. The mosaic nature of previously published subtype H strains VI557 and CA13 was reanalysed. MATERIALS AND METHODS: Peripheral blood mononuclear cells (PBMC) from individuals harbouring strains VI991 and VI997 were co-cultivated with PHA stimulated donor PBMC. Near-full-length genomes of VI991 and VI997, and gag and env genes of CA13 and VI557, were amplified by polymerase chain reaction, cloned and sequenced. Intersubtype recombination analyses were performed by similarity plot, bootscanning and phylogenetic analysis. RESULTS: Near-full-length clones of HIV-1 VI991 and VI997 are representative of subtype H. They form a phylogenetic cluster with the only previously described subtype H representative HIV-1 90CF056.1, regardless of the genome region analysed. VI557 is redefined as a gag and env subtype H mosaic virus containing unclassified fragments. CA13 is a complex intersubtype recombinant between subtypes A, H and unclassified strains CONCLUSION: Near-full-length genome analysis identified HIV-1 VI991 and VI997 as two new subtype H representatives. These reagents will allow defining and classifying non-recombinant as well as recombinant HIV-1, eventually helping to solve the puzzle of HIV-1 subtypes. en_US
dc.language English en_US
dc.publisher Lippincott, Williams & Wilkins
dc.subject HIV-1 en_US
dc.subject Virology en_US
dc.subject Genetics en_US
dc.subject Subtypes en_US
dc.title HIV-1 subtype H near-full length genome reference strains and analysis of subtype-H-containing inter-subtype recombinants en_US
dc.type Article en_US
dc.citation.jtitle AIDS en_US
dc.citation.volume 14 en_US
dc.citation.pages 1533-1543 en_US
dc.publisher.place Philadelphia
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/10983640
dc.citation.jabbreviation AIDS en_US


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