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A comparative evaluation of parasitological tests and a PCR for Trypanosoma evansi diagnosis in experimentally infected water buffaloes

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dc.contributor.author Holland, W. G. en_US
dc.contributor.author Claes, F. en_US
dc.contributor.author My, L. N. en_US
dc.contributor.author Thanh, N. G. en_US
dc.contributor.author Tam, P. T. en_US
dc.contributor.author Verloo, D. en_US
dc.contributor.author Büscher, P. en_US
dc.contributor.author Goddeeris, B. en_US
dc.contributor.author Vercruysse, J. en_US
dc.date.accessioned 2007-12-06T14:33:38Z
dc.date.available 2007-12-06T14:33:38Z
dc.date.issued 2001 en_US
dc.identifier.issn 0304-4017 en_US
dc.identifier.doi http://dx.doi.org/10.1016/S0304-4017(01)00381-8
dc.identifier.other ITG-P2A en_US
dc.identifier.other ITG-P6B en_US
dc.identifier.other ITG-P7A en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-SEROL en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/179
dc.description.abstract In this study five parasitological methods and a polymerase chain reaction (PCR) were compared for the diagnostic sensitivity for Trypanosoma evansi in experimentally infected water buffaloes over a period of 15 weeks. The combined estimates of sensitivity (CE(se)) of the PCR proved to be highest at 78.2%, closely followed by the mouse inoculation (MI), the micro-haematocrite centrifugation technique (MHCT) and the mini-anion-exchange centrifugation technique (MAECT) with CE(se) of, respectively, 74.0, 69.6 and 62.4%. The CE(se) of the buffy-coat technique (BCT) at 38.6% and the sodium dodecyl sulfate (SDS) clarification technique at 25.1% were considerably lower. PCR detected consistently all buffaloes infected from week 3 post-infection (PI) onwards. For MI this occurred after 5 weeks PI while for MHCT and MAECT these sustainable high levels were reached in the 7th week PI. BCT and SDS never detected all buffaloes infected. The influence of time and temperature on the viability of T. evansi in heparinized blood from water buffalo was also studied. In general we observed that the survival time tends to be longer when blood is kept at 4 degrees C. In samples kept in direct sunlight parasites became undetectable with the MHCT after 30min. After treatment of the water buffaloes with diminazene aceturate, the PCR signal disappeared within 24h. en_US
dc.language English en_US
dc.publisher Elsevier
dc.subject Protozoal diseases en_US
dc.subject Trypanosoma evansi en_US
dc.subject Buffaloes en_US
dc.subject Diagnosis en_US
dc.subject Tests en_US
dc.subject PCR en_US
dc.subject Evaluation en_US
dc.title A comparative evaluation of parasitological tests and a PCR for Trypanosoma evansi diagnosis in experimentally infected water buffaloes en_US
dc.type Article en_US
dc.citation.issue 1 en_US
dc.citation.jtitle Veterinary Parasitology en_US
dc.citation.volume 97 en_US
dc.citation.pages 23-33 en_US
dc.publisher.place Amsterdam
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/11337124
dc.identifier.url http://www.elsevier.com/locate/vetpar
dc.citation.jabbreviation Vet Parasitol en_US


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