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Resazurin microtiter assay plate testing of Mycobacterium tuberculosis susceptibilities to second-line drugs: rapid, simple, and inexpensive method

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dc.contributor.author Martin, A. en_US
dc.contributor.author Camacho, M. en_US
dc.contributor.author Portaels, F. en_US
dc.contributor.author Palomino, J. C. en_US
dc.date.accessioned 2007-12-06T14:47:30Z
dc.date.available 2007-12-06T14:47:30Z
dc.date.issued 2003 en_US
dc.identifier.issn 0066-4804 en_US
dc.identifier.doi http://dx.doi.org/10.1128/AAC.47.11.3616-3619.2003
dc.identifier.other ITG-M1B en_US
dc.identifier.other ITG-M3A en_US
dc.identifier.other ITG-MLA en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-MYCOB en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/1850
dc.description.abstract The emergence of multidrug-resistant tuberculosis calls for new, rapid drug susceptibility tests. We have tested 150 Mycobacterium tuberculosis isolates against the second-line drugs ethionamide, kanamycin, capreomycin, ofloxacin, and para-aminosalicylic acid by the colorimetric resazurin microtiter assay and the proportion method. By visual reading, MICs were obtained after 8 days. A very good correlation between results by the colorimetric resazurin microtiter assay and the proportion method was obtained. The colorimetric resazurin microtiter assay is inexpensive, rapid, and simple to perform, and implementation of the assay is feasible for low-resource countries. en_US
dc.language English en_US
dc.subject Bacterial diseases en_US
dc.subject Tuberculosis en_US
dc.subject Mycobacterium tuberculosis en_US
dc.subject Drug resistance en_US
dc.subject Second-line drugs en_US
dc.subject Drug sensitivity testing en_US
dc.subject Laboratory techniques and procedures en_US
dc.subject Evaluation en_US
dc.title Resazurin microtiter assay plate testing of Mycobacterium tuberculosis susceptibilities to second-line drugs: rapid, simple, and inexpensive method en_US
dc.type Article en_US
dc.citation.issue 11 en_US
dc.citation.jtitle Antimicrobial Agents and Chemotherapy en_US
dc.citation.volume 47 en_US
dc.citation.pages 3616-3619 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/14576129
dc.citation.jabbreviation Antimicrob Agents Chemother en_US


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