Institute of Tropical Medicine Antwerp
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Multicenter evaluation of the nitrate reductase assay for drug resistance detection of Mycobacterium tuberculosis

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dc.contributor.author Martin, A. en_US
dc.contributor.author Montoro, E. en_US
dc.contributor.author Lemus, D. en_US
dc.contributor.author Simboli, N. en_US
dc.contributor.author Morcillo, N. en_US
dc.contributor.author Valesco, M. en_US
dc.contributor.author Chauca, J. en_US
dc.contributor.author Barrera, L. en_US
dc.contributor.author Ritacco, V. en_US
dc.contributor.author Portaels, F. en_US
dc.contributor.author Palomino, J. C. en_US
dc.date.accessioned 2007-12-06T14:47:31Z
dc.date.available 2007-12-06T14:47:31Z
dc.date.issued 2005 en_US
dc.identifier.issn 0167-7012 en_US
dc.identifier.doi http://dx.doi.org/10.1016/j.mimet.2005.03.004
dc.identifier.other ITG-M1A en_US
dc.identifier.other ITG-M10A en_US
dc.identifier.other ITG-MLA en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-MYCOB en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/1851
dc.description.abstract The performance of the nitrate reductase assay was evaluated in a multicenter laboratory study to detect resistance of Mycobacterium tuberculosis to the first-line anti-tuberculosis drugs rifampicin, isoniazid, ethambutol and streptomycin using a set of coded isolates. Compared with the gold standard proportion method on Löwenstein-Jensen medium, the assay was highly accurate in detecting resistance to rifampicin, isoniazid and ethambutol with an accuracy of 98%, 96.6% and 97.9%, respectively. For streptomycin, discrepant results were obtained with an overall accuracy of 85.3%. The assay proved easy to be implemented in countries with limited laboratory facilities. en_US
dc.language English en_US
dc.subject Bacterial diseases en_US
dc.subject Mycobacterial diseases en_US
dc.subject Tuberculosis en_US
dc.subject Drug resistance en_US
dc.subject Drug sensitivity testing en_US
dc.subject Assays en_US
dc.subject Evaluation en_US
dc.title Multicenter evaluation of the nitrate reductase assay for drug resistance detection of Mycobacterium tuberculosis en_US
dc.type Article en_US
dc.citation.jtitle Journal of Microbiological Methods en_US
dc.citation.volume 63 en_US
dc.citation.pages 145-150 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/15893391
dc.citation.jabbreviation J Microbiol Meth en_US


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