| dc.contributor.author |
Martin, A. |
en_US |
| dc.contributor.author |
Montoro, E. |
en_US |
| dc.contributor.author |
Lemus, D. |
en_US |
| dc.contributor.author |
Simboli, N. |
en_US |
| dc.contributor.author |
Morcillo, N. |
en_US |
| dc.contributor.author |
Valesco, M. |
en_US |
| dc.contributor.author |
Chauca, J. |
en_US |
| dc.contributor.author |
Barrera, L. |
en_US |
| dc.contributor.author |
Ritacco, V. |
en_US |
| dc.contributor.author |
Portaels, F. |
en_US |
| dc.contributor.author |
Palomino, J. C. |
en_US |
| dc.date.accessioned |
2007-12-06T14:47:31Z |
|
| dc.date.available |
2007-12-06T14:47:31Z |
|
| dc.date.issued |
2005 |
en_US |
| dc.identifier.issn |
0167-7012 |
en_US |
| dc.identifier.doi |
http://dx.doi.org/10.1016/j.mimet.2005.03.004 |
|
| dc.identifier.other |
ITG-M1A |
en_US |
| dc.identifier.other |
ITG-M10A |
en_US |
| dc.identifier.other |
ITG-MLA |
en_US |
| dc.identifier.other |
MICRO |
en_US |
| dc.identifier.other |
U-MYCOB |
en_US |
| dc.identifier.other |
JIF |
en_US |
| dc.identifier.other |
DOI |
en_US |
| dc.identifier.other |
ABSTRACT |
en_US |
| dc.identifier.uri |
http://hdl.handle.net/10390/1851 |
|
| dc.description.abstract |
The performance of the nitrate reductase assay was evaluated in a multicenter laboratory study to detect resistance of Mycobacterium tuberculosis to the first-line anti-tuberculosis drugs rifampicin, isoniazid, ethambutol and streptomycin using a set of coded isolates. Compared with the gold standard proportion method on Löwenstein-Jensen medium, the assay was highly accurate in detecting resistance to rifampicin, isoniazid and ethambutol with an accuracy of 98%, 96.6% and 97.9%, respectively. For streptomycin, discrepant results were obtained with an overall accuracy of 85.3%. The assay proved easy to be implemented in countries with limited laboratory facilities. |
en_US |
| dc.language |
English |
en_US |
| dc.subject |
Bacterial diseases |
en_US |
| dc.subject |
Mycobacterial diseases |
en_US |
| dc.subject |
Tuberculosis |
en_US |
| dc.subject |
Drug resistance |
en_US |
| dc.subject |
Drug sensitivity testing |
en_US |
| dc.subject |
Assays |
en_US |
| dc.subject |
Evaluation |
en_US |
| dc.title |
Multicenter evaluation of the nitrate reductase assay for drug resistance detection of Mycobacterium tuberculosis |
en_US |
| dc.type |
Article |
en_US |
| dc.citation.jtitle |
Journal of Microbiological Methods |
en_US |
| dc.citation.volume |
63 |
en_US |
| dc.citation.pages |
145-150 |
en_US |
| dc.identifier.pmid |
http://www.ncbi.nlm.nih.gov/pubmed/15893391 |
|
| dc.citation.jabbreviation |
J Microbiol Meth |
en_US |