Institute of Tropical Medicine Antwerp
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A new rapid and simple colorimetric method to detect pyrazinamide resistance in Mycobacterium tuberculosis using nicotinamide

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dc.contributor.author Martin, A. en_US
dc.contributor.author Takiff, H. en_US
dc.contributor.author Vandamme, P. en_US
dc.contributor.author Swings, J. en_US
dc.contributor.author Palomino, J. C. en_US
dc.contributor.author Portaels, F. en_US
dc.date.accessioned 2007-12-06T14:47:32Z
dc.date.available 2007-12-06T14:47:32Z
dc.date.issued 2006 en_US
dc.identifier.issn 0305-7453 en_US
dc.identifier.doi http://dx.doi.org/10.1093/jac/dkl231
dc.identifier.other ITG-M1A en_US
dc.identifier.other ITG-M5A en_US
dc.identifier.other ITG-MLA en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-MYCOB en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/1855
dc.description.abstract OBJECTIVES: The purpose of this study was to develop and assess a rapid method for pyrazinamide resistance detection in Mycobacterium tuberculosis using nicotinamide in a colorimetric resazurin assay. METHODS: We have tested M. tuberculosis isolates using nicotinamide in a 96-well format with the redox indicator resazurin (REMA) and compared results using the BACTEC 460-TB system with two concentrations of pyrazinamide (100 and 300 mg/L), as well as the Wayne method for detecting pyrazinamidase activity. Mutations in the pncA gene were detected by DNA sequencing of the pyrazinamide-resistant strains. RESULTS: Out of 95 clinical isolates of M. tuberculosis tested, 25 were determined to be resistant by the Wayne, BACTEC (300 mg/L), and the REMA nicotinamide methods. Using a nicotinamide MIC>250 mg/L as the cut-off for defining resistance, only one strain was falsely labelled as resistant. The REMA nicotinamide assay demonstrated a sensitivity of 100% and a specificity of 98%. The BACTEC (100 mg/L) falsely classified 8 strains as resistant. DNA sequencing detected mutations in 18/22 of the pncA genes from pyrazinamide-resistant strains. CONCLUSIONS: The REMA plate using nicotinamide to detect resistance to pyrazinamide is a simple and rapid method that could be useful in limited-resource countries. en_US
dc.language English en_US
dc.subject Bacterial diseases en_US
dc.subject Tuberculosis en_US
dc.subject Mycobacterium tuberculosis en_US
dc.subject Drug resistance en_US
dc.subject First-line drugs en_US
dc.subject Resazurin en_US
dc.subject Pyrazinamide en_US
dc.subject Drug sensitivity testing en_US
dc.subject Rapid assessment en_US
dc.title A new rapid and simple colorimetric method to detect pyrazinamide resistance in Mycobacterium tuberculosis using nicotinamide en_US
dc.type Article en_US
dc.citation.issue 2 en_US
dc.citation.jtitle Journal of Antimicrobial Chemotherapy en_US
dc.citation.volume 58 en_US
dc.citation.pages 327-331 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/16751203
dc.citation.jabbreviation J Antimicrob Chemother en_US


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