Institute of Tropical Medicine Antwerp
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Serological detection of infection with diverse human and simian immunodeficiency viruses using consensus env peptides

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Show simple item record Masciotra, S. en_US Rudolph, D. L. en_US van der Groen, G. en_US Yang, C. en_US Lal, R. B. en_US 2007-12-06T14:47:33Z 2007-12-06T14:47:33Z 2000 en_US
dc.identifier.other ITG-M3A en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-VIROL en_US
dc.identifier.other JIF en_US
dc.identifier.other ABSTRACT en_US
dc.description.abstract Cross-species transmission has been shown to play an important role in the emergence of human retroviruses. We developed a generic enzyme immunoassay using synthetic peptides from gp41 and C2V3 consensus sequences (human immunodeficiency virus [HIV] type 1 [HIV-1] groups M, O, and N and the homologous region of simian immunodeficiency virus [SIV] strains from chimpanzees [SIVcpz], SIVcpzGAB1 and SIVcpzANT) to detect divergent HIV and SIV. A cocktail of peptides from gp41 and C2V3 (M-O) detected all HIV-1 group M and O sera and showed cross-reactivity with SIVcpz sera. Further, a mixture of C2V3 peptides (GAB1-ANT) failed to detect HIV-1 infections but reacted with all SIVcpz sera, allowing discrimination of SIVcpz from HIV-1 infections. Since most SIVcpz sera cross-reacted with HIV-1 peptides, we next evaluated SIVcpz serum reactivity with rapid tests for HIV-1/2. SIVcpzANT and SIVcpzUS sera reacted with the Sero-strip and Multispot assays. Both tests are sensitive in detecting group M (97 100%, respectively), although Multispot has lower sensitivity for group O detection (67%) than does Sero-strip (100%). The limited volume and time required to perform these assays make them a generic tool for field screening. The env peptide-based assay and rapid tests should allow for the identification of emerging variants of HIV and SIV. en_US
dc.language English en_US
dc.subject Viral diseases en_US
dc.subject HIV en_US
dc.subject SIV en_US
dc.subject Peptides en_US
dc.subject env en_US
dc.title Serological detection of infection with diverse human and simian immunodeficiency viruses using consensus env peptides en_US
dc.type Article en_US
dc.citation.issue 4 en_US
dc.citation.jtitle Clinical and Diagnostic Laboratory Immunology en_US
dc.citation.volume 7 en_US
dc.citation.pages 706-709 en_US
dc.citation.jabbreviation Clin Diagn Lab Immunol en_US

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