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Diagnostic performance of the Roche AMPLICOR PCR in detecting Neisseria gonorrhoeae in genitourinary specimens from female sex workers in Cotonou, Benin

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dc.contributor.author Mukenge-Tshibaka, L. en_US
dc.contributor.author Alary, M. en_US
dc.contributor.author Bernier, F. en_US
dc.contributor.author Van Dyck, E. en_US
dc.contributor.author Lowndes, C. M. en_US
dc.contributor.author Guédou, A. en_US
dc.contributor.author Anagonou, S. en_US
dc.contributor.author Joly, J. R. en_US
dc.date.accessioned 2007-12-06T14:47:38Z
dc.date.available 2007-12-06T14:47:38Z
dc.date.issued 2000 en_US
dc.identifier.issn 0095-1137 en_US
dc.identifier.other ITG-M4A en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-HIVSTD en_US
dc.identifier.other JIF en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/1871
dc.description.abstract The objective of this study was to evaluate the diagnostic performance of the Roche multiplex AMPLICOR Chlamydia trachomatis/Neisseria gonorrhoeae PCR test for the detection of Neisseria gonorrhoeae infection in female urine specimens and wet and dry endocervical swabs. Endocervical swabs and urine specimens were collected from 342 female sex workers from Cotonou, Benin, and were tested using the AMPLICOR C. trachomatis/N. gonorrhoeae test (Roche Diagnostic Systems, Inc., Branchburg, N.J.) with internal control detection. Endocervical swabs were also cultured on Thayer-Martin medium. A series of alternate standards that included a combination of all the tests but not the test being evaluated was used to assess the performance of the test with each type of specimen. The sensitivity, specificity, and positive and negative predictive values for the urine were 53.8, 98.9, 93.5, and 87.5%, respectively. Corresponding figures for the wet swab were 91.5, 100, 100, and 97.4%, respectively. Those for the dry swab were 96.3, 96.2, 88.5, and 98.8%, respectively. Based on this study, the AMPLICOR PCR assay showed a low sensitivity for detection of N. gonorrhoeae infection in urine specimens, whereas the test was found to be highly sensitive and specific with endocervical specimens. en_US
dc.language English en_US
dc.subject Sexually transmitted diseases en_US
dc.subject STD en_US
dc.subject Neisseria gonorrhoeae en_US
dc.subject Bacterial diseases en_US
dc.subject Diagnosis en_US
dc.subject Tests en_US
dc.subject Prostitutes en_US
dc.title Diagnostic performance of the Roche AMPLICOR PCR in detecting Neisseria gonorrhoeae in genitourinary specimens from female sex workers in Cotonou, Benin en_US
dc.type Article en_US
dc.citation.jtitle Journal of Clinical Microbiology en_US
dc.citation.volume 38 en_US
dc.citation.pages 4076-4079 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/11060071
dc.citation.jabbreviation J Clin Microbiol en_US


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