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A multiplex PCR-based method derived from random amplified polymorphic DNA (RAPD) markers for the identification of species of the Anopheles minimus group in Southeast Asia

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dc.contributor.author Kengne, P. en_US
dc.contributor.author Trung, H. D. en_US
dc.contributor.author Baimai, V. en_US
dc.contributor.author Coosemans, M. en_US
dc.contributor.author Manguin, S. en_US
dc.date.accessioned 2007-12-06T14:33:41Z
dc.date.available 2007-12-06T14:33:41Z
dc.date.issued 2001 en_US
dc.identifier.issn 0962-1075 en_US
dc.identifier.other ITG-P4A en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-ENTOM en_US
dc.identifier.other JIF en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/187
dc.description.abstract Effective control of Anopheles minimus s.l., an important malaria vector in Southeast Asia, is based on the accurate identification of species within An. minimus complex, which cannot be distinguished using morphological characters. Derived from individual random amplified polymorphic DNA markers, sequence characterized amplified regions were analysed for the design of species-specific paired-primers. Combination of these primers resulted in the development of a simple, robust multiplex PCR able to identify both species An. minimus A and C belonging to the complex, hybrids AC, and three sympatric and closely related species, An. aconitus, An. pampanai and An. varuna. Hybrids AC do not possess alleles of both parents but exhibit novel adaptive potentials resulting from recombination among parental genes leading to hybrizyme. en_US
dc.language English en_US
dc.subject Parasitology en_US
dc.subject Anopheles minimus en_US
dc.subject Identification en_US
dc.subject Asia, Southeast en_US
dc.title A multiplex PCR-based method derived from random amplified polymorphic DNA (RAPD) markers for the identification of species of the Anopheles minimus group in Southeast Asia en_US
dc.type Article en_US
dc.citation.jtitle Insect Molecular Biology en_US
dc.citation.volume 10 en_US
dc.citation.pages 427-435 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/11881807
dc.citation.jabbreviation Insect Mol Biol en_US


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