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Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis

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dc.contributor.author Rigouts, L. en_US
dc.contributor.author Nolasco, O. en_US
dc.contributor.author de Rijk, P. en_US
dc.contributor.author Nduwamahoro, E. en_US
dc.contributor.author Van Deun, A. en_US
dc.contributor.author Ramsay, A. en_US
dc.contributor.author Arevalo, J. en_US
dc.contributor.author Portaels, F. en_US
dc.date.accessioned 2007-12-06T14:48:03Z
dc.date.available 2007-12-06T14:48:03Z
dc.date.issued 2007 en_US
dc.identifier.issn 0095-1137 en_US
dc.identifier.doi http://dx.doi.org/10.1128/JCM.01489-06
dc.identifier.other ITG-M1A en_US
dc.identifier.other ITG-M3B en_US
dc.identifier.other ITG-M4B en_US
dc.identifier.other ITG-M5A en_US
dc.identifier.other ITG-MLA en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-MYCOB en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/1934
dc.description.abstract New rpoB gene primers for detecting Rif(r) in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanol-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rif(r) isolates examined, 97% had mutations within cluster I, 2% at codon 176, and 1% at codon 497. en_US
dc.language English en_US
dc.publisher American Society for Microbiology en_US
dc.subject Bacterial diseases en_US
dc.subject Tuberculosis en_US
dc.subject Mycobacterium tuberculosis en_US
dc.subject Rifampin en_US
dc.subject Drug sensitivity testing en_US
dc.subject Primers en_US
dc.subject Sensitivity en_US
dc.subject Specificity en_US
dc.subject Efficacy en_US
dc.subject Laboratory techniques and procedures en_US
dc.title Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis en_US
dc.type Article en_US
dc.citation.issue 1 en_US
dc.citation.jtitle Journal of Clinical Microbiology en_US
dc.citation.volume 45 en_US
dc.citation.pages 252-254 en_US
dc.publisher.place Washington en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/17093024
dc.citation.jabbreviation J Clin Microbiol en_US


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