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Evaluation of the resazurin assay for the detection of multidrug-resistant Mycobacterium tuberculosis in Madagascar

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Show simple item record Rivoire, N. en_US Ravololonandriana, P. en_US Rasolonavalona, T. en_US Martin, A. en_US Portaels, F. en_US Ramarokoto, H. en_US Razanamparany, V. R. en_US 2007-12-06T14:48:03Z 2007-12-06T14:48:03Z 2007 en_US
dc.identifier.issn 1027-3719 en_US
dc.identifier.other ITG-M4A en_US
dc.identifier.other ITG-M5A en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-MYCOB en_US
dc.identifier.other JIF en_US
dc.identifier.other ABSTRACT en_US
dc.description.abstract SETTING: Multidrug-resistant (MDR) tuberculosis (TB) can jeopardise the success of national TB control programmes. Rapid, simple drug susceptibility tests applicable in developing countries would allow earlier treatment of patients with MDR infections. OBJECTIVE: To test the feasibility and performance of the resazurin microtitre assay (REMA) as an indirect test for detecting isoniazid (INH) and rifampicin (RMP) resistance of Mycobacterium tuberculosis strains in Madagascar. DESIGN: Study comparing the sensitivity and specificity of the REMA plate test with the Löwenstein-Jensen proportion method for determining the resistance of M. tuberculosis strains to INH and RMP. RESULTS: The sensitivity and specificity of the resazurin test were studied in 77 strains and were respectively 95% and 97.3% for the detection of INH resistance, and 95% and 100% for the detection of RMP resistance. The sensitivity and specificity for the identification of MDR strains were respectively 89% and 100%. CONCLUSION: The resazurin test is sensitive and specific enough for the detection of INH- and RMP-resistant strains. It is also easy to use, rapid and inexpensive, making it suitable for developing countries. Its usefulness for national drug resistance surveys should be assessed.
dc.language English en_US
dc.publisher International Union Against Tuberculosis And Lung Disease
dc.subject Bacterial diseases en_US
dc.subject Tuberculosis en_US
dc.subject Multidrug resistance en_US
dc.subject Drug sensitivity testing en_US
dc.subject Rapid assessment en_US
dc.subject Resazurin en_US
dc.subject Feasibility en_US
dc.subject Performance en_US
dc.subject Madagascar en_US
dc.subject Africa, East en_US
dc.subject Indian Ocean en_US
dc.title Evaluation of the resazurin assay for the detection of multidrug-resistant Mycobacterium tuberculosis in Madagascar en_US
dc.type Article en_US
dc.citation.issue 6 en_US
dc.citation.jtitle International Journal of Tuberculosis and Lung Disease en_US
dc.citation.volume 11 en_US
dc.citation.pages 683-688 en_US Paris
dc.citation.jabbreviation Int J Tuberc Lung Dis en_US

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