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A simple PCR method for rapid genotype analysis of Mycobacterium ulcerans

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Show simple item record Stinear, T. en_US Davies, J. K. en_US Jenkin, G. A. en_US Portaels, F. en_US Ross, B. C. en_US Oppedisano, F. en_US Purcell, M. en_US Hayman, J. A. en_US 2007-12-06T14:48:16Z 2007-12-06T14:48:16Z 2000 en_US
dc.identifier.issn 0095-1137 en_US
dc.identifier.other ITG-M4A en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-MYCOB en_US
dc.identifier.other JIF en_US
dc.identifier.other ABSTRACT en_US
dc.description.abstract Two high-copy-number insertion sequences, IS2404 and IS2606, were recently identified in Mycobacterium ulcerans and were shown by Southern hybridization to possess restriction fragment length polymorphism between strains from different geographic origins. We have designed a simple genotyping method that captures these differences by PCR amplification of the region between adjacent copies of IS2404 and IS2606. We have called this system 2426 PCR. The method is rapid, reproducible, sensitive, and specific for M. ulcerans, and it has confirmed previous studies suggesting a clonal population structure of M. ulcerans within a geographic region. M. ulcerans isolates from Australia, Papua New Guinea, Malaysia, Surinam, Mexico, Japan, China, and several countries in Africa were easily differentiated based on an array of 4 to 14 PCR products ranging in size from 200 to 900 bp. Numerical analysis of the banding patterns suggested a close evolutionary link between M. ulcerans isolates from Africa and southeast Asia. The application of 2426 PCR to total DNA, extracted directly from M. ulcerans-infected tissue specimens without culture, demonstrated the sensitivity and specificity of this method and confirmed for the first time that both animal and human isolates from areas of endemicity in southeast Australia have the same genotype. en_US
dc.language English en_US
dc.publisher American Society for Microbiology en_US
dc.subject Bacterial diseases en_US
dc.subject Mycobacterium ulcerans en_US
dc.subject Genotyping en_US
dc.subject PCR en_US
dc.title A simple PCR method for rapid genotype analysis of Mycobacterium ulcerans en_US
dc.type Article en_US
dc.citation.jtitle Journal of Clinical Microbiology en_US
dc.citation.volume 38 en_US
dc.citation.pages 1482-1487 en_US Washington en_US
dc.citation.jabbreviation J Clin Microbiol en_US

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