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Genotyping Mycobacterium ulcerans and Mycobacterium marinum by using mycobacterial interspersed repetitive units

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dc.contributor.author Stragier, P. en_US
dc.contributor.author Ablordey, A. en_US
dc.contributor.author Meyers, W. M. en_US
dc.contributor.author Portaels, F. en_US
dc.date.accessioned 2007-12-06T14:48:17Z
dc.date.available 2007-12-06T14:48:17Z
dc.date.issued 2005 en_US
dc.identifier.issn 0021-9193 en_US
dc.identifier.doi http://dx.doi.org/10.1128/JB.187.5.1639-1647.2005
dc.identifier.other ITG-M1B en_US
dc.identifier.other ITG-M2B en_US
dc.identifier.other ITG-MLA en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-MYCOB en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/1969
dc.description.abstract A novel category of variable tandem repeats (VNTR) called mycobacterial interspersed repetitive units (MIRUs) has been identified for Mycobacterium ulcerans (n = 39), M. marinum (n = 27), and one related organism. Fifteen MIRU loci were identified in the genome of M. marinum and were used to genotype M. ulcerans, M. marinum, and an M. marinum-like organism that is considered a possible missing link between M. marinum and M. ulcerans. Seven MIRU loci were polymorphic, and locus-specific PCRs for four of these loci differentiated seven M. ulcerans genotypes, four M. marinum genotypes, and a unique genotype for the missing link organism. The seven M. ulcerans genotypes were related to six different geographic origins of isolates. All isolates from West and Central Africa, including old and recent isolates, belonged to the same genotype, emphasizing the great spatiotemporal homogeneity among African isolates. Unlike the M. ulcerans genotypes, the four M. marinum genotypes could not be clearly related to the geographic origins of the isolates. According to MIRU-VNTR typing, all M. ulcerans and M. marinum isolates of American origin were closely related, suggesting a common American ancestor for these two pathogenic species on the American continents. MIRU typing has significant potential value for discriminating between reoccurrence and reinfection for M. ulcerans disease. en_US
dc.language English en_US
dc.subject Bacteriology en_US
dc.subject Mycobacterium ulcerans en_US
dc.subject Mycobacterium marinum en_US
dc.subject Characterization en_US
dc.subject Genotyping en_US
dc.subject MIRU en_US
dc.subject Reinfection en_US
dc.subject Reoccurrence en_US
dc.title Genotyping Mycobacterium ulcerans and Mycobacterium marinum by using mycobacterial interspersed repetitive units en_US
dc.type Article en_US
dc.citation.issue 5 en_US
dc.citation.jtitle Journal of Bacteriology en_US
dc.citation.volume 187 en_US
dc.citation.pages 1639-1647 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/15716434
dc.citation.jabbreviation J Bacteriol en_US


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