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Rapid identification of mycobacteria to the species level using INNO-LiPA mycobacteria, a reverse hybridization assay

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Show simple item record Suffys, P. N. en_US da Silva Rocha, A. en_US de Oliveira, M. en_US Dias Campos, C. E. en_US Werneck Barreto, A. M. en_US Portaels, F. en_US Rigouts, L. en_US Wouters, G. en_US Jannes, G. en_US Van Reybroeck, G. en_US Mijs, W. en_US Vanderborght, B. en_US 2007-12-06T14:48:17Z 2007-12-06T14:48:17Z 2001 en_US
dc.identifier.issn 0095-1137 en_US
dc.identifier.other ITG-M6A en_US
dc.identifier.other ITG-M7A en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-MYCOB en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.description.abstract INNO-LiPA Mycobacteria (LiPA; Innogenetics, Zwijnaarde, Belgium) is a kit for the simultaneous detection and identification of Mycobacterium species in culture and identifies the Mycobacterium tuberculosis complex, the M. avium complex (MAC), and the following Mycobacterium species: M. kansasii, M. avium, M. intracellulare, M. scrofulaceum, M. gordonae, M. xenopi, and the M. chelonae-M. abscessus complex. The assay, which targets the 16S-23S rRNA spacer region, was evaluated on 157 mycobacterial strains that had been identified by conventional techniques and PCR-restriction enzyme analysis of the hsp65 gene (PRA). Forty-seven reference strains consisting of 37 different species and 110 human clinical isolates were submitted to the test, and all were hybridized with the Mycobacterium genus probe (MYC) on the LiPA strip (100% sensitivity). Ninety-four isolates hybridized to their corresponding species- or complex-specific probes; only one isolate phenotypically identified as M. gordonae did not react with its specific probe (99.4% accuracy). Thirty-seven MAC strains were phenotypically identified to the complex level and to the species level by LiPA as M. avium (n = 18) or M. intracellulare (n = 7) or as belonging to the M. avium-M. intracellulare-M. scrofulaceum complex (n = 12). Of the last 12 strains, 10 had M. avium PRA patterns and 2 had M. intracellulare PRA patterns. Three isolates that had been identified as a single species by conventional identification were proven to be mixed cultures by the LiPA assay. The whole procedure can be performed in 1 working day, starting with the supernatant of a small amount of bacterial mass that had been treated by freezing and then boiling. en_US
dc.language English en_US
dc.publisher American Society for Microbiology en_US
dc.subject Bacteriology en_US
dc.subject Mycobacteria en_US
dc.subject Identification en_US
dc.subject Species en_US
dc.subject Assays en_US
dc.subject LiPA en_US
dc.title Rapid identification of mycobacteria to the species level using INNO-LiPA mycobacteria, a reverse hybridization assay en_US
dc.type Article en_US
dc.citation.issue 12 en_US
dc.citation.jtitle Journal of Clinical Microbiology en_US
dc.citation.volume 39 en_US
dc.citation.pages 4477-4482 en_US Washington en_US
dc.citation.jabbreviation J Clin Microbiol en_US

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