Institute of Tropical Medicine Antwerp
Foundation of Public Utility

National external quality assessment scheme for lymphocyte immunophenotyping in Belgium

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Show simple item record Van Blerk, M. en_US Bernier, M. en_US Bossuyt, X. en_US Chatelain, B. en_US D'Hautcourt, J. L. en_US Demanet, C. en_US Kestens, L. en_US Van Bockstaele, D. en_US Crucitti, T. en_US Libeer, J. C. en_US 2007-12-06T14:48:25Z 2007-12-06T14:48:25Z 2003 en_US
dc.identifier.issn 1434-6621 en_US
dc.identifier.other ITG-NOT en_US
dc.identifier.other ITG-N7M en_US
dc.identifier.other ITG-N9M en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-IMMUN en_US
dc.identifier.other U-VIROL en_US
dc.identifier.other JIF en_US
dc.identifier.other ADN en_US
dc.identifier.other ABSTRACT en_US
dc.description.abstract In 2000, the Belgian Scientific Institute of Public Health introduced a voluntary external quality assessment scheme for lymphocyte immunophenotyping. This paper provides an analysis of the first six surveys. Specimens consisted of fresh EDTA-anticoagulated whole blood and were sent by overnight mail. The 41 participants were surveyed for methodology and were asked to report white blood cell count, percentage of lymphocytes, and percentages and absolute numbers of CD3+, CD4+, CD8+, and CD19+ cells. Median intralaboratory coefficients of variation were 1.0, 1.3, 1.7, and 3.2% for CD3+, CD4+, CD8+, and CD19+ cell percentages, respectively. Interlaboratory variability was consistently lower than 6.5% for CD3+ and CD4+CD3+ cell percentages, and lower than 9.5% for CD8+CD3+ cell percentages. Median coefficients of variation for the absolute values were higher, ranging from 10.1% for CD4+CD3+ cells to 16.5% for CD19+ cells. The percentage of CD4+CD3+ and CD8+ CD3+ cells was in several samples significantly lower than the percentage of total CD4+ and CD8+ cells. The number of laboratories measuring total CD4+ and CD8+ cells decreased by 30% during the programme. Between-laboratory variability remained stable over time. Analysis of individual laboratory performance indicated that some laboratories markedly improved their results. en_US
dc.language English en_US
dc.subject Laboratory techniques and procedures en_US
dc.subject Quality assurance en_US
dc.subject Flow cytometry en_US
dc.subject T lymphocytes en_US
dc.subject B lymphocytes en_US
dc.subject Quantification en_US
dc.subject Phenotypes en_US
dc.subject Quality assessment en_US
dc.title National external quality assessment scheme for lymphocyte immunophenotyping in Belgium en_US
dc.type Article en_US
dc.citation.jtitle Clinical Chemistry and Laboratory Medicine en_US
dc.citation.volume 41 en_US
dc.citation.pages 323-330 en_US
dc.citation.jabbreviation Clin Chem Lab Med en_US

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