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Performance of commercially available enzyme immunoassays for detection of antibodies against herpes simplex virus type 2 in African populations

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dc.contributor.author Van Dyck, E. en_US
dc.contributor.author Buvé, A. en_US
dc.contributor.author Weiss, H. A. en_US
dc.contributor.author Glynn, J. R. en_US
dc.contributor.author Brown, D. W. G. en_US
dc.contributor.author De Deken, B. en_US
dc.contributor.author Parry, J. en_US
dc.contributor.author Hayes, R. J. en_US
dc.date.accessioned 2007-12-06T14:48:41Z
dc.date.available 2007-12-06T14:48:41Z
dc.date.issued 2004 en_US
dc.identifier.issn 0095-1137 en_US
dc.identifier.doi http://dx.doi.org/10.1128/JCM.42.7.2961-2965.2004
dc.identifier.other ITG-M1A en_US
dc.identifier.other ITG-M2A en_US
dc.identifier.other ITG-M6B en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-HIVSTD en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/2032
dc.description.abstract Data are accumulating on the performance of enzyme immunoassays (EIAs) for the detection of herpes simplex virus type 2 (HSV-2) infection in North America and Europe, but little is known about their performance in other populations. Nine test kits were evaluated with 330 serum samples from sub-Saharan Africa. The tests were first compared to the monoclonal antibody (MAb) EIA (Central Public Health Laboratory, London, United Kingdom). Samples that gave discordant results in the MAb EIA and in the three tests that performed best compared to the MAb EIA were tested by Western blotting (University of Washington, Seattle). A random sample of concordant samples was also tested, and the sensitivities and specificities of the different tests were calculated, taking into account this sampling strategy. The sensitivities of the tests ranged from 86 to 100%; the specificities ranged from 47 to 99%. The tests that performed best were the Gull Premier EIA (sensitivity, 86.3%; specificity, 97.6%) and the Kalon Biological (sensitivity, 92.3%; specificity, 97.7%) and Biokit (sensitivity, 86.7%; specificity, 92.6%) tests. It cannot be assumed that enzyme immunoassays for the detection of HSV-2 infection that perform well in industrialized countries will perform equally well in other populations. en_US
dc.language English en_US
dc.publisher American Society for Microbiology en_US
dc.subject Viral diseases en_US
dc.subject Herpes simplex virus type 2 en_US
dc.subject Serodiagnosis en_US
dc.subject Antibody detection en_US
dc.subject Immunoenzyme techniques en_US
dc.subject Laboratory techniques and procedures en_US
dc.subject Comparative study en_US
dc.subject Africa en_US
dc.title Performance of commercially available enzyme immunoassays for detection of antibodies against herpes simplex virus type 2 in African populations en_US
dc.type Article en_US
dc.citation.issue 7 en_US
dc.citation.jtitle Journal of Clinical Microbiology en_US
dc.citation.volume 42 en_US
dc.citation.pages 2961-2965 en_US
dc.publisher.place Washington en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/15243045
dc.citation.jabbreviation J Clin Microbiol en_US


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