Institute of Tropical Medicine Antwerp
Foundation of Public Utility

Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by enzyme immunoassay, culture, and three nucleic acid amplification tests

DSpace/Manakin Repository

Show simple item record

dc.contributor.author Van Dyck, E. en_US
dc.contributor.author Ieven, M. en_US
dc.contributor.author Pattyn, S. en_US
dc.contributor.author Van Damme, L. en_US
dc.contributor.author Laga, M. en_US
dc.date.accessioned 2007-12-06T14:48:41Z
dc.date.available 2007-12-06T14:48:41Z
dc.date.issued 2001 en_US
dc.identifier.issn 0095-1137 en_US
dc.identifier.doi http://dx.doi.org/10.1128/JCM.39.5.1751-1756.2001
dc.identifier.other ITG-M1A en_US
dc.identifier.other ITG-M3A en_US
dc.identifier.other ITG-M4A en_US
dc.identifier.other ITG-MLA en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-HIVSTD en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/2034
dc.description.abstract The purpose of this study was to evaluate and compare three commercially available nucleic acid amplification tests (NAATs) for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis. Roche PCR and Becton Dickinson strand displacement amplification (SDA) were performed on 733 endocervical swab specimens from commercial sex workers. Abbott ligase chain reaction (LCR) was performed on a subset of 396 samples. Endocervical specimens from all women were also tested by culture for N. gonorrhoeae and by Syva MicroTrak enzyme immunoassay (EIA) for C. trachomatis. A positive N. gonorrhoeae result was defined as a positive result by culture or by two NAATs, and a positive C. trachomatis result was defined as a positive result by two tests. According to these definitions, the sensitivities and specificities for the subsample of 396 specimens of N. gonorrhoeae culture, PCR, SDA, and LCR were 69.8, 95.2, 88.9, and 88.9% and 100, 99.4, 100, and 99.1%, respectively; the sensitivities and specificities of C. trachomatis EIA, PCR, SDA, and LCR were 42.0, 98.0, 94.0, and 90.0% and 100, 98.0, 100, and 98.6%, respectively. The performance characteristics of N. gonorrhoeae culture, PCR, and SDA and C. trachomatis EIA, PCR, and SDA for all 733 specimens were defined without inclusion of LCR results and by discrepant analysis after resolution of discordant N. gonorrhoeae PCR results and of discordant C. trachomatis EIA and PCR results by LCR testing. The sensitivities of N. gonorrhoeae culture, PCR, and SDA before and after LCR resolution were 67.8, 95.7, and 93.9% and 65, 95.8, and 90.0%, respectively. The sensitivities of C. trachomatis EIA, PCR, and SDA decreased from 39.4, 100, and 100% to 38.7, 98.7, and 94.7%, respectively. All three NAATs proved to be superior to N. gonorrhoeae culture and to C. trachomatis EIA. The accuracies of the different NAATs were quite similar. SDA was the only amplification assay with 100% specificity for detection of both N. gonorrhoeae and C. trachomatis in endocervical specimens. en_US
dc.language English en_US
dc.publisher American Society for Microbiology en_US
dc.subject Sexually transmitted diseases en_US
dc.subject STD en_US
dc.subject Chlamydia trachomatis en_US
dc.subject Viral diseases en_US
dc.subject Neisseria gonorrhoeae en_US
dc.subject Bacterial diseases en_US
dc.subject Detection en_US
dc.subject Laboratories en_US
dc.subject Tests en_US
dc.title Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by enzyme immunoassay, culture, and three nucleic acid amplification tests en_US
dc.type Article en_US
dc.citation.issue 5 en_US
dc.citation.jtitle Journal of Clinical Microbiology en_US
dc.citation.volume 39 en_US
dc.citation.pages 1751-1756 en_US
dc.publisher.place Washington en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/11325985
dc.citation.jabbreviation J Clin Microbiol en_US


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record