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Towards saliva-based screening for sleeping sickness?

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dc.contributor.author Lejon, V. en_US
dc.contributor.author Kwete, J. en_US
dc.contributor.author Büscher, P. en_US
dc.date.accessioned 2007-12-06T14:33:45Z
dc.date.available 2007-12-06T14:33:45Z
dc.date.issued 2003 en_US
dc.identifier.issn 1360-2276 en_US
dc.identifier.doi http://dx.doi.org/10.1046/j.1365-3156.2003.01077.x
dc.identifier.other ITG-P1A en_US
dc.identifier.other ITG-P3A en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-SEROL en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other FTB en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/203
dc.description The definitive version is available at www3.interscience.wiley.com
dc.description.abstract A pilot study was carried out on the detection of trypanosome-specific antibodies in saliva for diagnosis of sleeping sickness. All twenty-three saliva samples of parasitologically confirmed Trypanosoma brucei gambiense patients tested positive in an indirect enzyme-linked immunosorbent assay, whereas all 14 saliva samples of a negative control group remained negative. Trypanosome-specific antibody levels in patient saliva correlated with antibody levels in serum, but were about 250-fold lower. Eight of 23 undiluted saliva samples of sleeping sickness patients tested positive in CATT/T. b. gambiense and two of 23 in LATEX/T. b. gambiense. All fourteen saliva samples of the negative control group were also positive in CATT/T. b. gambiense, as were four of 14 in LATEX/T. b. gambiense. CATT and LATEX were thus inappropriate for antibody detection in saliva. These results indicate that trypanosome-specific antibody detection in saliva is possible. This could lead to the development of a simple, non-invasive, reliable saliva field test for diagnosis of sleeping sickness. en_US
dc.language English en_US
dc.publisher Blackwell Publishing
dc.subject Protozoal diseases en_US
dc.subject Trypanosomiasis, African en_US
dc.subject Trypanosoma brucei gambiense en_US
dc.subject Case detection en_US
dc.subject Laboratory diagnosis en_US
dc.subject Antibody detection en_US
dc.subject Saliva en_US
dc.subject ELISA en_US
dc.subject CATT en_US
dc.subject LATEX en_US
dc.title Towards saliva-based screening for sleeping sickness? en_US
dc.type Article en_US
dc.citation.issue 7
dc.citation.jtitle Tropical Medicine and International Health en_US
dc.citation.volume 8 en_US
dc.citation.pages 585-588 en_US
dc.publisher.place Oxford
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/12828539
dc.citation.jabbreviation Trop Med Int Health en_US


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