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Modeling HIV transfer between dendritic cells and T cells: importance of HIV phenotype, dendritic cell-T cell contact and T-cell activation

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dc.contributor.author Vanham, G. en_US
dc.contributor.author Penne, L. en_US
dc.contributor.author Allemeersch, H. en_US
dc.contributor.author Kestens, L. en_US
dc.contributor.author Willems, B. en_US
dc.contributor.author van der Groen, G. en_US
dc.contributor.author Jeang, K. T. en_US
dc.contributor.author Toossi, Z. en_US
dc.contributor.author Rich, E. en_US
dc.date.accessioned 2007-12-06T14:48:44Z
dc.date.available 2007-12-06T14:48:44Z
dc.date.issued 2000 en_US
dc.identifier.issn 0269-9370 en_US
dc.identifier.other ITG-M1A en_US
dc.identifier.other ITG-M2B en_US
dc.identifier.other ITG-M3B en_US
dc.identifier.other ITG-M4A en_US
dc.identifier.other ITG-M5B en_US
dc.identifier.other ITG-M6A en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-IMMUN en_US
dc.identifier.other U-VIROL en_US
dc.identifier.other JIF en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/2041
dc.description Not the final published version en_US
dc.description.abstract OBJECTIVE: To study the requirements for HIV transfer between dendritic cells (DC) and CD4 T cells, using an in vitro model, combined with flow cytometry. METHODS: Immature DC and macrophages (MA) were generated from monocytes. After infection, DC or MA were cultured alone or with purified CD4 T cells. Intracellular HIV was measured, using (1) the monocyte (MO)-tropic AD8 HIV, endowed with enhanced green fluorescent protein (EGFP); and (2) intracellular staining of laboratory HIV strains and clones from primary isolates. RESULTS: (1) Clone AD8-EGFP infected DC and MA with equal efficiency, but the virus was preferentially transferred from DC to autologous T cells. (2) DC were more productively infected with R5/NSI, as compared to X4/SI, HIV, but both HIV phenotypes were easily transmitted to autologous T4 cells. (3) HIV-infected DC transferred the virus to T cells across a semi-permeable membrane, if the T cells were in contact with non-infected DC. (4) Co-culture of T cells with autologous non-infected DC induced T-cell activation. HIV-infected DC selectively increased HLA-DR on T cells and HLA-DR (+) T cells were preferential targets for HIV transfer. (5) Resting Ba-L-infected CD4 T cells were able to transmit the virus 'inversely' to co-cultured DC. CONCLUSION: HIV transfer between monocyte-derived dendritic cells and autologous CD4 T cells was directly demonstrated using flow cytometry. The transfer proceeded in both directions, depended on cellular contact and was associated with partial T-cell activation. This model, representing relevant in vivo targets of HIV, is useful to further investigate interactions between HIV, DC and T cells, without the need for primary ex vivo DC. en_US
dc.language English en_US
dc.publisher Lippincott, Williams & Wilkins
dc.subject Virology en_US
dc.subject HIV en_US
dc.subject Dendritic cells en_US
dc.subject T-cells en_US
dc.subject Transfer en_US
dc.subject Phenotypes en_US
dc.title Modeling HIV transfer between dendritic cells and T cells: importance of HIV phenotype, dendritic cell-T cell contact and T-cell activation en_US
dc.type Article en_US
dc.citation.jtitle AIDS en_US
dc.citation.volume 14 en_US
dc.citation.pages 2299-2311 en_US
dc.publisher.place Philadelphia
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/11089618
dc.citation.jabbreviation AIDS en_US


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