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Detection of diverse variants of human immunodeficiency virus-1 groups M, N, and O and simian immunodeficiency viruses from chimpanzees by using generic pol and env primer pairs

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dc.contributor.author Yang, C. en_US
dc.contributor.author Dash, B. C. en_US
dc.contributor.author Simon, F. en_US
dc.contributor.author van der Groen, G. en_US
dc.contributor.author Pieniazek, D. en_US
dc.contributor.author Gao, F. en_US
dc.contributor.author Hahn, B. H. en_US
dc.contributor.author Lal, R. B. en_US
dc.date.accessioned 2007-12-06T14:48:55Z
dc.date.available 2007-12-06T14:48:55Z
dc.date.issued 2000 en_US
dc.identifier.issn 0022-1899 en_US
dc.identifier.doi http://dx.doi.org/10.1086/315439
dc.identifier.other ITG-M4A en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-VIROL en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other FTA en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/2067
dc.description.abstract Human immunodeficiency virus type 1 (HIV-1) infection of humans is the result of independent cross-species transmissions of simian immunodeficiency viruses (SIVcpz) from naturally infected chimpanzees (Pan troglodytes troglodytes) to man. To develop a polymerase chain reaction-based assay capable of detecting members of all major phylogenetic SIVcpz and HIV-1 lineages (groups M, N, and O), primer pairs in conserved pol and env regions were designed. Both primer sets amplified </=10 copies of selected group M reference clones (subtypes A-H), proviral DNA or RNA of group N (YBF30), and group O of HIV-1 and also amplified divergent SIVcpz from cultured isolates (SIVcpzGAB1 and SIVcpzANT), uncultured spleen tissue (SIVcpzUS), and plasma (SIVcpzANT and SIVcpzUS). Sequences of the 2 amplicons (445 bp for gp41 and 261 bp for integrase) are of sufficient length for phylogenetic analyses, allowing both group and subtype classifications of the human viruses. Finally, both primer pairs are highly sensitive (>99%) in amplifying viral sequences from plasma taken from patients infected with HIV-1 group M (n=226) and O (n=17) viruses. en_US
dc.language English en_US
dc.publisher University of Chicago Press en_US
dc.subject Virology en_US
dc.subject Detection en_US
dc.subject HIV-1 group M en_US
dc.subject HIV-1 group N en_US
dc.subject HIV-1 group O en_US
dc.subject SIV en_US
dc.subject env en_US
dc.subject pol en_US
dc.title Detection of diverse variants of human immunodeficiency virus-1 groups M, N, and O and simian immunodeficiency viruses from chimpanzees by using generic pol and env primer pairs en_US
dc.type Article en_US
dc.citation.issue 5 en_US
dc.citation.jtitle Journal of Infectious Diseases en_US
dc.citation.volume 181 en_US
dc.citation.pages 1791-1795 en_US
dc.publisher.place Chicago en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/10823786
dc.citation.jabbreviation J Infect Dis en_US


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