Institute of Tropical Medicine Antwerp
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Human African trypanosomiasis: quantitative and qualitative assessment of intrathecal immune response

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Show simple item record Lejon, V. en_US Sindic, C. J. M. en_US Van Antwerpen, M. P. en_US Doua, F. en_US Solano, P. en_US Jamonneau, V. en_US Wouters, I. en_US Büscher, P. en_US 2007-12-06T14:33:47Z 2007-12-06T14:33:47Z 2003 en_US
dc.identifier.issn 1351-5101 en_US
dc.identifier.other ITG-P1A en_US
dc.identifier.other ITG-P8B en_US
dc.identifier.other ITG-PLA en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-SEROL en_US
dc.identifier.other JIF en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.other FTC
dc.description.abstract Quantitative and qualitative techniques for assessment of the intrathecal humoral immune response in human African trypanosomiasis were compared, and their diagnostic potential for detection of the meningo-encephalitic stage of the disease was evaluated. Total and trypanosome specific immunoglobulin G (IgG) and IgM intrathecal synthesis were studied in paired cerebrospinal fluid (CSF) and blood samples of 38 trypanosomiasis patients and in three controls using Reiber's formulae. The presence of CSF-specific oligoclonal IgG and of trypanosome-specific antibodies was determined using iso-electric focusing followed by immunoblotting and antigen-driven immunoblots. The intrathecal IgG fraction (16% positive) and oligoclonal IgG detection (24% positive) were insensitive for detection of an intrathecal humoral immune response. Trypanosome-specific IgG synthesis, reflected by the IgG antibody index (AI) (26% positive), was confirmed by the presence of oligoclonal specific IgG (47% positive), but the latter was more sensitive. Although the detection technique failed for oligoclonal IgM, the intrathecal IgM fraction (42% positive) and the IgM AI (32% positive) indicated that the meningo-encephalitic stage of the disease is characterized by a dominant intrathecal IgM response, which was higher than the IgG response. The highest combination of diagnostic sensitivity and specificity for the meningo-encephalitic stage of trypanosomiasis was observed for quantitative IgM determinations. en_US
dc.language English en_US
dc.subject Protozoal diseases en_US
dc.subject Trypanosomiasis, African en_US
dc.subject Intrathecal humoral immune response en_US
dc.subject Intrathecal immunoglobulin synthesis en_US
dc.subject Assessment en_US
dc.subject Qualitative en_US
dc.subject Quantitative en_US
dc.subject Cerebrospinal fluid en_US
dc.subject Blood en_US
dc.title Human African trypanosomiasis: quantitative and qualitative assessment of intrathecal immune response en_US
dc.type Article en_US
dc.citation.jtitle European Journal of Neurology en_US
dc.citation.volume 10 en_US
dc.citation.pages 711-719 en_US
dc.citation.jabbreviation Eur J Neurol en_US

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