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Human African trypanosomiasis: quantitative and qualitative assessment of intrathecal immune response

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dc.contributor.author Lejon, V. en_US
dc.contributor.author Sindic, C. J. M. en_US
dc.contributor.author Van Antwerpen, M. P. en_US
dc.contributor.author Doua, F. en_US
dc.contributor.author Solano, P. en_US
dc.contributor.author Jamonneau, V. en_US
dc.contributor.author Wouters, I. en_US
dc.contributor.author Büscher, P. en_US
dc.date.accessioned 2007-12-06T14:33:47Z
dc.date.available 2007-12-06T14:33:47Z
dc.date.issued 2003 en_US
dc.identifier.issn 1351-5101 en_US
dc.identifier.other ITG-P1A en_US
dc.identifier.other ITG-P8B en_US
dc.identifier.other ITG-PLA en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-SEROL en_US
dc.identifier.other JIF en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.other FTC
dc.identifier.uri http://hdl.handle.net/10390/211
dc.description.abstract Quantitative and qualitative techniques for assessment of the intrathecal humoral immune response in human African trypanosomiasis were compared, and their diagnostic potential for detection of the meningo-encephalitic stage of the disease was evaluated. Total and trypanosome specific immunoglobulin G (IgG) and IgM intrathecal synthesis were studied in paired cerebrospinal fluid (CSF) and blood samples of 38 trypanosomiasis patients and in three controls using Reiber's formulae. The presence of CSF-specific oligoclonal IgG and of trypanosome-specific antibodies was determined using iso-electric focusing followed by immunoblotting and antigen-driven immunoblots. The intrathecal IgG fraction (16% positive) and oligoclonal IgG detection (24% positive) were insensitive for detection of an intrathecal humoral immune response. Trypanosome-specific IgG synthesis, reflected by the IgG antibody index (AI) (26% positive), was confirmed by the presence of oligoclonal specific IgG (47% positive), but the latter was more sensitive. Although the detection technique failed for oligoclonal IgM, the intrathecal IgM fraction (42% positive) and the IgM AI (32% positive) indicated that the meningo-encephalitic stage of the disease is characterized by a dominant intrathecal IgM response, which was higher than the IgG response. The highest combination of diagnostic sensitivity and specificity for the meningo-encephalitic stage of trypanosomiasis was observed for quantitative IgM determinations. en_US
dc.language English en_US
dc.subject Protozoal diseases en_US
dc.subject Trypanosomiasis, African en_US
dc.subject Intrathecal humoral immune response en_US
dc.subject Intrathecal immunoglobulin synthesis en_US
dc.subject Assessment en_US
dc.subject Qualitative en_US
dc.subject Quantitative en_US
dc.subject Cerebrospinal fluid en_US
dc.subject Blood en_US
dc.title Human African trypanosomiasis: quantitative and qualitative assessment of intrathecal immune response en_US
dc.type Article en_US
dc.citation.jtitle European Journal of Neurology en_US
dc.citation.volume 10 en_US
dc.citation.pages 711-719 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/14641518
dc.citation.jabbreviation Eur J Neurol en_US


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