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PCR approach for the detection of Trypanosoma brucei and T. equiperdum and their differentiation from T. evansi based on maxicircle kinetoplast DNA

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dc.contributor.author Li, F. J. en_US
dc.contributor.author Gasser, R. B. en_US
dc.contributor.author Lai, D. H. en_US
dc.contributor.author Claes, F. en_US
dc.contributor.author Zhu, X. Q. en_US
dc.contributor.author Lun, Z. R. en_US
dc.date.accessioned 2007-12-06T14:33:48Z
dc.date.available 2007-12-06T14:33:48Z
dc.date.issued 2007 en_US
dc.identifier.issn 0890-8508 en_US
dc.identifier.doi http://dx.doi.org/10.1016/j.mcp.2006.03.009
dc.identifier.other ITG-P4A en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-SEROL en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/212
dc.description.abstract The goal of this study was to develop a PCR approach based on the sequence of maxicircle kinetoplast DNA (kDNA) of Trypanosoma brucei to distinguish T. brucei/T. equiperdum from T. evansi and to evaluate its diagnostic use for their detection in blood samples. Primers derived from the sequence of the maxicircle kDNA of T. brucei, encoding the NADH dehydrogenase subunit 5 (nad5) gene, were used to test the PCR-amplification from T. brucei (including T. b. brucei and T. b. rhodesiense), T. equiperdum, T. evansi, T. vivax and T. congolense. A primer pair to a nuclear DNA region incorporated into a separate PCR was employed to control for the presence of amplifiable genomic DNA (representing the subgenus Trypanozoon) in each sample subjected to the PCR. Products of approximately 395bp were amplified from all T. brucei and T. equiperdum samples tested using the nad5-PCR, but not from T. evansi DNA samples or any of the control samples representing T. vivax, T. congolense, or host. The current PCR approach allows the rapid differentiation of T. brucei/T.equiperdum from T. evansi and can detect the equivalent of 20-25 cells of T. brucei or T. equiperdum in purified genomic DNA or infected blood samples. en_US
dc.language English en_US
dc.subject Protozoology en_US
dc.subject Trypanosoma evansi en_US
dc.subject Trypanosoma equiperdum en_US
dc.subject Trypanosoma brucei en_US
dc.subject Identification en_US
dc.subject Differentiation en_US
dc.subject Laboratory diagnosis en_US
dc.subject PCR en_US
dc.subject Kinetoplast DNA en_US
dc.subject Maxicircle en_US
dc.subject Laboratory techniques and procedures en_US
dc.title PCR approach for the detection of Trypanosoma brucei and T. equiperdum and their differentiation from T. evansi based on maxicircle kinetoplast DNA en_US
dc.type Article en_US
dc.citation.issue 1 en_US
dc.citation.jtitle Molecular and Cellular Probes en_US
dc.citation.volume 21 en_US
dc.citation.pages 1-7 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/16806809
dc.citation.jabbreviation Mol Cell Probes en_US


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