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Application of multiple DNA fingerprinting techniques to study the genetic relationships among three members of the subgenus Trypanozoon (Protozoa: Trypanosomatidae)

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Show simple item record Li, F. J. en_US Gasser, R. B. en_US Zheng, J. Y. en_US Claes, F. en_US Zhu, X. Q. en_US Lun, Z. R. en_US 2007-12-06T14:33:48Z 2007-12-06T14:33:48Z 2005 en_US
dc.identifier.issn 0890-8508 en_US
dc.identifier.other ITG-P4A en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-SEROL en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.description.abstract Three different DNA fingerprinting techniques, the mobile genetic element (MGE)-PCR, simple sequence repeat (SSR)-PCR and random amplified polymorphic DNA (RAPD)-PCR, were used to define a large set of genetic markers to study genetic similarity within and among Trypanosoma brucei, Trypanosoma equiperdum and Trypanosoma evansi strains (n=18) from China, Africa and South America and to investigate their genetic relationships. Using the three fingerprinting techniques, >890 bands (ranging in size from 0.2 to 2kb) were defined for all 18 strains of Trypanosoma. Within each of the strains, 39-59 bands were defined. The similarity coefficients between strains ranged from approximately 41 to 94%, with a mean of 65%. There was more genetic similarity among strains within T. evansi (mean of approximately 79%) compared with T. equiperdum ( approximately 65%) and T. brucei ( approximately 59%). The similarity coefficient data were used to construct the dendrogram, which revealed that (irrespective of species) the majority of strains from China and South America grouped together to the exclusion of those from Africa. The exceptions were a T. brucei strain from Africa and a T. equiperdum strain of unknown origin. Hence, employing data sets generated using the three different fingerprinting methods, it was not possible to unequivocally distinguish among T. brucei, T. evansi and T. equiperdum, although there was a tendency for T. evansi strains to group together to the exclusion of T. brucei. The findings provide support for the hypothesis that T. evansi originated from a mutated form of T. equiperdum and stimulate further investigations of the genetic make-up and evolution of members of the subgenus Trypanozoon. en_US
dc.language English en_US
dc.subject Protozoology en_US
dc.subject Trypanosomatids en_US
dc.subject Trypanozoon en_US
dc.subject Genetic diversity en_US
dc.subject DNA fingerprinting en_US
dc.subject Laboratory techniques and procedures en_US
dc.title Application of multiple DNA fingerprinting techniques to study the genetic relationships among three members of the subgenus Trypanozoon (Protozoa: Trypanosomatidae) en_US
dc.type Article en_US
dc.citation.issue 6 en_US
dc.citation.jtitle Molecular and Cellular Probes en_US
dc.citation.volume 19 en_US
dc.citation.pages 400-407 en_US
dc.citation.jabbreviation Mol Cell Probes en_US

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