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Primary culture of Mycobacterium ulcerans from human tissue specimens after storage in semisolid transport medium

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dc.contributor.author Eddyani, M. en_US
dc.contributor.author Debacker, M. en_US
dc.contributor.author Martin, A. en_US
dc.contributor.author Aguiar, J. en_US
dc.contributor.author Johnson, C. R. en_US
dc.contributor.author Uwizeye, C. en_US
dc.contributor.author Fisette, K. en_US
dc.contributor.author Portaels, F. en_US
dc.date.accessioned 2008-03-13T12:27:24Z
dc.date.available 2008-03-13T12:27:24Z
dc.date.issued 2008
dc.identifier.issn 0095-1137
dc.identifier.doi http://dx.doi.org/10.1128/JCM.00301-07
dc.identifier.other ITG-M1B en_US
dc.identifier.other ITG-M2B en_US
dc.identifier.other ITG-M3A en_US
dc.identifier.other ITG-M6B en_US
dc.identifier.other ITG-M7B en_US
dc.identifier.other ITG-MLA en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-MYCOB en_US
dc.identifier.other JIF en_US
dc.identifier.other UPD1 en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/2169
dc.description.abstract Tissue specimens collected from patients with clinically suspected Buruli ulcer treated in two Buruli ulcer treatment centers in Benin between 1998 and 2004 were placed in semisolid transport medium and transported at ambient temperature for microbiological analysis at the Institute of Tropical Medicine in Antwerp, Belgium. The impact of the delay before microbiological analysis on primary culture of Mycobacterium ulcerans was investigated. The length of storage in semisolid transport medium varied from 6 days to 26 weeks. Of the 1,273 tissue fragments positive for M. ulcerans DNA by an IS2404-specific PCR, 576 (45.2%) yielded positive culture results. The sensitivity of direct smear examination was 64.6% (822/1,273 tissue fragments). The median time required to obtain a positive culture result was 11 weeks. Positive cultures were obtained even from samples kept for more than 2 months at ambient temperatures. Moreover, there was no reduction in the viability of M. ulcerans, as detected by culture, when specimens remained in semisolid transport medium for long periods of time (up to 26 weeks). We can conclude that the method with semisolid transport medium is very robust for clinical specimens from patients with Buruli ulcer that, due to circumstances, cannot be analyzed in a timely manner. This transport medium is thus very useful for the confirmation of a diagnosis of Buruli ulcer with specimens collected in the field. en_US
dc.language English en_US
dc.publisher American Society for Microbiology en_US
dc.subject Bacterial diseases en_US
dc.subject Mycobacterium ulcerans en_US
dc.subject Buruli ulcer en_US
dc.subject Specimen transportation en_US
dc.title Primary culture of Mycobacterium ulcerans from human tissue specimens after storage in semisolid transport medium en_US
dc.type Article en_US
dc.citation.issue 1 en_US
dc.citation.jtitle Journal of Clinical Microbiology en_US
dc.citation.volume 46 en_US
dc.citation.pages 69-72 en_US
dc.publisher.place Washington en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/17989199
dc.citation.jabbreviation J Clin Microbiol en_US


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