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Effects of grinding surgical tissue specimens and smear staining methods on Buruli ulcer microscopic diagnosis

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Show simple item record Affolabi, D. en_US Bankolé, H. en_US Ablordey, A. en_US Hounnouga, J. en_US Koutchakpo, P. en_US Sopoh, G. en_US Aguiar, J. en_US Dossou, A. en_US Johnson, R. C. en_US Anagonou, S. en_US Portaels, F. en_US 2008-04-07T11:03:39Z 2008-04-07T11:03:39Z 2008
dc.identifier.issn 1360-2276
dc.identifier.other ITG-M1B en_US
dc.identifier.other ITG-M2B en_US
dc.identifier.other ITG-MLA en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-MYCOB en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other UPD1 en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.other FTB
dc.description The definitive version is available at
dc.description.abstract To optimize Buruli ulcer (BU) microscopic diagnosis, we compared two smear preparation methods from tissue specimens: smears made with tissue suspension after grinding and smears made directly with unground tissue. We also compared two smear staining methods: auramine and Ziehl-Neelsen (ZN). IS 2404-PCR was used as reference method. One hundred and thirty-one surgical tissue specimens from patients suspected of having BU were analyzed. Both smear preparation methods and both staining methods were equivalent in any combination. Thus we recommend ZN stained smears of unground tissue for peripheral treatment centres. en_US
dc.language English en_US
dc.publisher Blackwell Publishing
dc.subject Bacterial diseases en_US
dc.subject Mycobacterium ulcerans en_US
dc.subject Buruli ulcer en_US
dc.subject Laboratory diagnosis en_US
dc.subject Microscopy en_US
dc.subject Smear en_US
dc.subject Tissue en_US
dc.subject Specimen processing en_US
dc.subject Staining en_US
dc.subject Grinding en_US
dc.title Effects of grinding surgical tissue specimens and smear staining methods on Buruli ulcer microscopic diagnosis en_US
dc.type Article en_US
dc.citation.issue 2 en_US
dc.citation.jtitle Tropical Medicine and International Health en_US
dc.citation.volume 13 en_US
dc.citation.pages 187-190 en_US Oxford
dc.citation.jabbreviation Trop Med Int Health en_US

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