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Effects of grinding surgical tissue specimens and smear staining methods on Buruli ulcer microscopic diagnosis

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dc.contributor.author Affolabi, D. en_US
dc.contributor.author Bankolé, H. en_US
dc.contributor.author Ablordey, A. en_US
dc.contributor.author Hounnouga, J. en_US
dc.contributor.author Koutchakpo, P. en_US
dc.contributor.author Sopoh, G. en_US
dc.contributor.author Aguiar, J. en_US
dc.contributor.author Dossou, A. en_US
dc.contributor.author Johnson, R. C. en_US
dc.contributor.author Anagonou, S. en_US
dc.contributor.author Portaels, F. en_US
dc.date.accessioned 2008-04-07T11:03:39Z
dc.date.available 2008-04-07T11:03:39Z
dc.date.issued 2008
dc.identifier.issn 1360-2276
dc.identifier.doi http://dx.doi.org/10.1111/j.1365-3156.2007.01989.x
dc.identifier.other ITG-M1B en_US
dc.identifier.other ITG-M2B en_US
dc.identifier.other ITG-MLA en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-MYCOB en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other UPD1 en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.other FTB
dc.identifier.uri http://hdl.handle.net/10390/2224
dc.description The definitive version is available at www3.interscience.wiley.com
dc.description.abstract To optimize Buruli ulcer (BU) microscopic diagnosis, we compared two smear preparation methods from tissue specimens: smears made with tissue suspension after grinding and smears made directly with unground tissue. We also compared two smear staining methods: auramine and Ziehl-Neelsen (ZN). IS 2404-PCR was used as reference method. One hundred and thirty-one surgical tissue specimens from patients suspected of having BU were analyzed. Both smear preparation methods and both staining methods were equivalent in any combination. Thus we recommend ZN stained smears of unground tissue for peripheral treatment centres. en_US
dc.language English en_US
dc.publisher Blackwell Publishing
dc.subject Bacterial diseases en_US
dc.subject Mycobacterium ulcerans en_US
dc.subject Buruli ulcer en_US
dc.subject Laboratory diagnosis en_US
dc.subject Microscopy en_US
dc.subject Smear en_US
dc.subject Tissue en_US
dc.subject Specimen processing en_US
dc.subject Staining en_US
dc.subject Grinding en_US
dc.title Effects of grinding surgical tissue specimens and smear staining methods on Buruli ulcer microscopic diagnosis en_US
dc.type Article en_US
dc.citation.issue 2 en_US
dc.citation.jtitle Tropical Medicine and International Health en_US
dc.citation.volume 13 en_US
dc.citation.pages 187-190 en_US
dc.publisher.place Oxford
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/18304264
dc.citation.jabbreviation Trop Med Int Health en_US


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