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Antiviral compounds show enhanced activity in HIV-1 single cycle pseudovirus assays as compared to classical PBMC assays

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dc.contributor.author Heyndrickx, L. en_US
dc.contributor.author Vermoesen, T. en_US
dc.contributor.author Vereecken, K. en_US
dc.contributor.author Kurth, J. en_US
dc.contributor.author Coppens, S. en_US
dc.contributor.author Aerts, L. en_US
dc.contributor.author Ohagen, A. en_US
dc.contributor.author Van Herrewege, Y. en_US
dc.contributor.author Lewi, P. en_US
dc.contributor.author Vanham, G. en_US
dc.date.accessioned 2008-05-19T14:41:21Z
dc.date.available 2008-05-19T14:41:21Z
dc.date.issued 2008
dc.identifier.issn English
dc.identifier.other ITG-M1A en_US
dc.identifier.other ITG-M2B en_US
dc.identifier.other ITG-M3B en_US
dc.identifier.other ITG-M5B en_US
dc.identifier.other ITG-M6B en_US
dc.identifier.other ITG-M8A en_US
dc.identifier.other ITG-MLA en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-VIROL en_US
dc.identifier.other JIF en_US
dc.identifier.other UPD3 en_US
dc.identifier.other CHECK-DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/2255
dc.description.abstract HIV-1 Env pseudotyped viruses (PV) are an attractive tool for studying the antiviral activities of compounds interfering with virus entry into a target cell. To investigate whether results obtained in PV assays are relevant biologically, the antiviral activity of 6 reference compounds was compared on 5 virus isolates of different clades using three assays: (1) replicating virus in peripheral blood mononuclear cells (PBMCs), (2) PV in CD4 and CCR5- or CXCR4 co-receptor expressing Ghost cells, and (3) PV in PBMCs. A significant linear relationship was found between both single-cycle PV assays (P<0.0001, R2=0.75). Moreover, both assays showed enhanced sensitivity to the antiretrovirals tested (P=0.013 and 0.015, respectively) as compared to the PBMC assay with replication-competent virus. Most importantly, results from the latter assay could be predicted significantly from both PV assays, in which either Ghost target cells (P<0.0001, R2=0.61) or PBMCs (P<0.0001, R2=0.55) were used. The usefulness of the PV assay was demonstrated further by investigating the impact of the HIV-1 Env subtype on the antiviral activity of five new compounds derived from the entry inhibitor BMS806. en_US
dc.language English en_US
dc.subject Viral diseases en_US
dc.subject HIV-1 en_US
dc.subject AIDS en_US
dc.subject Drug development en_US
dc.subject Pseudoviruses, HIV-1 en_US
dc.title Antiviral compounds show enhanced activity in HIV-1 single cycle pseudovirus assays as compared to classical PBMC assays en_US
dc.type Article en_US
dc.citation.issue 1-2 en_US
dc.citation.jtitle Journal of Virological Methods en_US
dc.citation.volume 148 en_US
dc.citation.pages 166-173 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/18192031
dc.citation.jabbreviation J Virol Methods en_US


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