Institute of Tropical Medicine Antwerp
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Development of a competitive ELISA for detection of Theileria annulata infection

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dc.contributor.author Renneker, S. en_US
dc.contributor.author Kullmann, B. en_US
dc.contributor.author Gerber, S. en_US
dc.contributor.author Dobschanski, J. en_US
dc.contributor.author Bakheit, M. A. en_US
dc.contributor.author Geysen, D. en_US
dc.contributor.author Shiels, B. en_US
dc.contributor.author Tait, A. en_US
dc.contributor.author Ahmed, J. S. en_US
dc.contributor.author Seitzer, U. en_US
dc.date.accessioned 2008-09-17T15:17:18Z
dc.date.available 2008-09-17T15:17:18Z
dc.date.issued 2008
dc.identifier.issn 1865-1674
dc.identifier.doi http://dx.doi.org/10.1111/j.1865-1682.2008.01036.x
dc.identifier.other ITG-A6A en_US
dc.identifier.other ANIMAL en_US
dc.identifier.other U-ANIMAL en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.other UPD6 en_US
dc.identifier.uri http://hdl.handle.net/10390/2365
dc.description.abstract In previous studies, Theileria annulata surface protein (TaSP) was identified as an immunodominant antigen and successfully used to develop and validate a recombinant-protein-based ELISA for the detection of circulating antibodies in serum of T. annulata–infected animals. In this study, the same antigen was used to develop a competitive ELISA (cELISA) using a monoclonal antibody that was found to bind to TaSP. The cELISA accurately differentiated T. annulata-infected from uninfected animals and demonstrated a satisfactory performance with a calculated sensitivity and specificity of 77.4% and 100%, respectively. Thus the test proved its suitability for the diagnosis of tropical theileriosis and has application for use in serological surveys to monitor the prevalence of the disease or identify carrier animals with high specificity. en_US
dc.language English en_US
dc.subject Animal diseases en_US
dc.subject Protozoal diseases en_US
dc.subject Theileriasis en_US
dc.subject Theileria annulata en_US
dc.subject Case detection en_US
dc.subject Laboratory diagnosis en_US
dc.subject ELISA en_US
dc.subject Surface protein antigens en_US
dc.title Development of a competitive ELISA for detection of Theileria annulata infection en_US
dc.type Article en_US
dc.citation.issue 5-6 en_US
dc.citation.jtitle Transboundary and Emerging Diseases en_US
dc.citation.volume 55 en_US
dc.citation.pages 249-256 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/18666970
dc.citation.jabbreviation Transbound Emerg Dis en_US


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